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在乙谜麻醉下,分别于明时(8:00 a.m.)及暗时(8:00 p.m.)断头处死野生型及组胺H1R基因敲除型小鼠,迅速取出脑组织并分离出皮层、纹状体、海马、下丘脑、丘脑、中脑及脑干等脑区.这些脑组织被制成匀浆并用HPLC荧光检测法测量其组胺含量.结果显示暗时处死时,H1R基因敲除型小鼠海马、丘脑、中脑及脑干中的组胺含量明显低于野生型小鼠.明时处死时,野生型小鼠各脑区组胺含量均较暗时处死显著降低,但这一变化在H1R基因敲除型小鼠中并未观察到.这些表明作为组胺的功能靶,H1R不仅介导组胺的功能,而且调节大脑中组胺含量与释放的昼夜节律.
Wild type and histamine H1R knockout mice were killed by decapitation in the Ming (8:00 am) and dark (8:00 pm) sessions, respectively. Brain tissues were removed and the cortex was isolated. Striatum, hippocampus, hypothalamus, thalamus, midbrain and brainstem etc. The brain tissue was homogenized and its histamine content was measured by HPLC fluorescence detection.The results showed that H1R knockout Histamine content in hippocampus, thalamus, midbrain and brainstem of mice was significantly lower than that of wild-type mice. A change was not observed in H1R knockout mice, suggesting that H1R, as a functional target of histamine, not only mediates the function of histamine, but also regulates the circadian rhythm of histamine content and release in the brain.