论文部分内容阅读
目的研究3,4-二氯苯胺(3,4-DCA)对雄性大鼠精子的影响,以探讨3,4-DCA对雄性大鼠的生殖毒性作用。方法将40只健康成年SPF级Wistar雄性大鼠按体重随机分为39、81、170、357 mg/kg 3,4-DCA染毒组和溶剂对照组(玉米油),每组8只,经口灌胃染毒,灌胃容积为5 ml/kg,1次/d,连续染毒35 d。染毒结束后,处死大鼠,取附睾制成精子悬液,测定精子密度;台盼蓝染色测定精子的存活率,观察精子的活动能力并分级,计算精子活动率;测定精子的畸形率。结果与对照组比较,39 mg/kg 3,4-DCA染毒组体重略有增加,但差异无统计学意义(P>0.05);与对照组比较,170、357 mg/kg 3,4-DCA染毒组体重降低,差异均有统计学意义(P<0.01)。各组大鼠睾丸系数和附睾系数比较,差异无统计学意义(P>0.05)。与对照组比较,170和357 mg/kg 3,4-DCA染毒组精子密度降低,81、170、357 mg/kg 3,4-DCA染毒组精子存活率和精子活动率降低,39、81、170和357 mg/kg 3,4-DCA染毒组精子畸形率升高,差异均有统计学意义(P<0.05或P<0.01)。结论3,4-DCA可能通过影响机体免疫功能以及抑制睾丸中重要酶的活力,最终影响精子生成的数量和质量。
Objective To study the effect of 3,4-dichloroaniline (3,4-DCA) on the sperm of male rats to explore the reproductive toxicity of 3,4-DCA on male rats. Methods Forty healthy adult Wistar male Spre-WF rats were randomly divided into 39,81,170,357 mg / kg 3,4-DCA and control group (corn oil) Oral stomach poisoning, gastric perfusion volume of 5 ml / kg, 1 / d, continuous exposure to 35 d. After the exposure, the rats were sacrificed, and the sperms were made into sperm suspension to measure the sperm density. The survival rate of spermatozoa was determined by trypan blue staining, the sperm motility was graded, the sperm motility was calculated, and the sperm deformity was measured. Results Compared with the control group, the body weight of 39 mg / kg 3,4-DCA increased slightly, but the difference was not statistically significant (P> 0.05). Compared with the control group, The body weight of DCA-treated group decreased significantly (P <0.01). There was no significant difference in testis coefficient and epididymis between the two groups (P> 0.05). Compared with the control group, the sperm concentration of 170 and 357 mg / kg 3,4-DCA groups decreased, while the sperm and sperm motility rates of 81, 170 and 357 mg / kg 3,4-DCA groups decreased, The sperm deformity rates of 81, 170 and 357 mg / kg 3,4-DCA groups were significantly increased (P <0.05 or P <0.01). Conclusion 3,4-DCA may affect the body’s immune function and inhibit the vitality of important enzymes in the testes, ultimately affecting the quantity and quality of spermatogenesis.