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对乔纳锦等苹果品种的茎尖或侧芽进行离体培养,在基本培养基附加BA1.0mg/L(或再加IAA0.1mg/L)的培养基上培养2个月左右可分化出丛生芽。丛生芽的嫩梢和丛生芽基部切块继代于基本培养基附加BA0.5～1.0mg/L(或再加少量IAA或NAA)不同激素处理的培养基上均能生长分化。用嫩梢继代培养获得的试管苗比较粗壮,培养过程中去顶可促进腋生芽的分化和生长。诱导生根效果较好的激素处理为IBA0.5mg/L+IAA0.3mg/L。
In vitro culture of shoot tips or lateral buds of apple varieties such as Jonathan, cultured on medium supplemented with basic BA1.0mg / L (or IAA0.1mg / L) medium for 2 months can differentiate into clusters bud. The shoots of shoots and buds at the base of clustered buds were able to grow and differentiate on medium supplemented with BA0.5-1.0 mg / L (or with a small amount of IAA or NAA) supplemented with different hormones. Subculture with tender shoots obtained relatively strong plantlets, to the top of the cultivation process can promote axillary bud differentiation and growth. The best hormones for inducing rooting were IBA0.5mg / L + IAA0.3mg / L.