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采用外源添加方式,通过监测藻细胞数量变化,分析了香草酸、对羟基苯甲酸和焦性没食子酸对中肋骨条藻生长的抑制作用。结果表明,在32~256mg/L浓度范围内,随着浓度的增大,3种酚酸对中肋骨条藻的抑制作用明显增强。当浓度为192mg/L时,3种酚酸对中肋骨条藻的生长抑制率超过50%。香草酸、对羟基苯甲酸和焦性没食子酸对中肋骨条藻的半抑制浓度分别为153mg/L、145mg/L和108.0mg/L。进一步,研究发现3种酚酸的半抑制浓度作用下,藻细胞内叶绿素、蛋白质和多糖含量明显低于对照组,胞外蛋白质和胞外多糖含量没有明显差异,但二者比值与对照组相比,有明显的增大趋势。3种酚酸显著降低了藻细胞SOD和POD比活力,尤其是显著降低了POD比活力,使其比活力降低为对照组的18%~39%。香草酸、对羟基苯甲酸和焦性没食子酸均致使藻细胞MDA含量明显升高,其数值增大为对照组的1.6、2.2和2.4倍。上述研究表明,3种酚酸通过影响某些主要生化成分的合成及其活性的影响,从而实现对中肋骨条藻生长的抑制作用。
The effects of vanillic acid, p-hydroxybenzoic acid and pyrogallic acid on the growth of Skeletonema costatum were analyzed by using exogenous ways to monitor the changes of the number of algal cells. The results showed that in the concentration range of 32 ~ 256mg / L, the inhibitory effect of the three phenolic acids on S. costatum was significantly enhanced with the increase of concentration. When the concentration of 192mg / L, the three kinds of phenolic acids on S. costatum growth inhibition rate of more than 50%. The half-inhibitory concentrations of vanillic acid, p-hydroxybenzoic acid and pyrogallol were 153 mg / L, 145 mg / L and 108.0 mg / L, respectively. Further, the results showed that the content of chlorophyll, protein and polysaccharide in algae cells was significantly lower than that in control group under the effect of half inhibitory concentration of three kinds of phenolic acids, while the content of extracellular protein and extracellular polysaccharide did not differ significantly. However, Than the obvious increase trend. The three kinds of phenolic acids significantly reduced the specific activity of SOD and POD of algal cells, especially significantly reduced the specific activity of POD, reducing its specific activity to 18% -39% of the control. Vanillic acid, p-hydroxybenzoic acid and pyrogallol both caused the MDA content in algal cells to increase significantly, which increased to 1.6, 2.2 and 2.4 times of the control. The above studies show that the three kinds of phenolic acids can affect the growth of Skeletonema costatum by affecting the synthesis and activity of some major biochemical components.