喉癌中抗凋亡基因bcl-2蛋白的表达及其临床意义

• 作 者: 刘克杰 刘大煜 王惠忠
• 机 构: 山东临沂医学专科学校!276002,山东医科大学附属医院,山东省淄博侨联医院
• 出 处: 泰山医学院学报
• 发表日期: 1999年03期
• 关键词: bcl-2蛋白 肿癌 免疫组织化学 抗凋亡基因 细胞凋亡抑制 肿瘤分化程度 病理组织学 肿瘤复发 乳腺癌预后 细胞恶变

目的　探讨细胞凋亡抑制基因ｂｃｌ－ ２ 蛋白产物在喉癌中的表达及其临床意义。方法　应用免疫组化ＳＡＢＣ方法检测４１ 例喉癌切除标本石蜡切片组织。结果　ｂｃｌ－ ２ 蛋白在喉癌组织中有过度表达，其阳性表达率为５１２ ％ （２４／４１） ；ｂｃｌ－ ２ 表达与喉癌病理组织学分级相关，肿瘤分化程度越高，ｂｃｌ－ ２ 阳性表过率越高（ Ｐ＜ ００５） ，早期（ Ⅰ、Ⅱ期） 喉癌中ｂｃｌ－ ２ 表达明显高于晚期（ Ⅲ、Ⅳ期）（ Ｐ＜００５） ；ｂｃｌ－ ２ 表达与肿瘤复发、转移无关Ｐ＞ ００５） ；而与预后明显相关，ｂｃｌ－ ２ 阳性组三年生存率明显高于阴性组Ｐ＜ ００５） 。结论　ｂｃｌ－ ２ 基因在喉癌中有异常表达，与喉癌的发生、发展相关，并可作为判断其预后的指标之一

Objective To investigate the expression of bcl-2 protein in human laryngeal squamous cell carcinoma and its clinical significance. Methods Immunohistochemical SABC method was used to detect the paraffin sections of 41 laryngeal cancer specimens. Results The bcl-2 protein was overexpressed in laryngeal squamous cell carcinoma with a positive rate of 512% (24/41). The expression of bcl-2 was correlated with the pathological grade of laryngeal carcinoma. The higher the degree of tumor differentiation was, the higher the bcl- (P <005). The expression of bcl-2 in laryngeal carcinoma was higher than that in advanced stage (Ⅲ, Ⅳ) (P <005), bcl-2 Expression had no correlation with tumor recurrence and metastasis (P> 0.05), but significantly correlated with prognosis. The three-year survival rate of bcl-2 positive group was significantly higher than that of negative group (P <005). Conclusions The bcl-2 gene is abnormally expressed in laryngeal carcinoma, which is related to the occurrence and development of laryngeal carcinoma and may be used as one of the prognostic indicators