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以往认为慢性化脓性中耳炎的主要致病菌是葡萄球菌、绿脓杆菌及变形杆菌等。近年来L 型菌的致病性逐渐被引起重视。我们采取30例慢性化脓性中耳炎的脓液进行 L 型菌培养,阳性率达83.2%,现报告如下。一、一般资料:30例中男16例,女14例,年龄10～72岁,患中耳炎史2～30年。二、检查方法:消毒外耳道后用消毒棉扦取中耳脓液接种于 L 型培养基,置35℃、5.6%CO_2孵箱内培养24～48小时。观察菌落为灰白色,细小。在8×10低倍镜下挑“油煎蛋”状或颗粒透明的菌落作革氏染色,见染色各异、形态不一的杆状巨形体和大小不等的卵园形园球体。作细胞壁染色发现胞壁缺损定为L 型菌。挑 L 型菌落转种普通琼脂平板35℃、CO_2孵箱培养返祖,鉴定其菌株。
In the past that the main pathogens of chronic suppurative otitis media is Staphylococcus aureus, Pseudomonas aeruginosa and Proteus and so on. In recent years, the pathogenicity of L-type bacteria gradually attracted attention. We take 30 cases of chronic suppurative otitis media pus L-type culture, the positive rate of 83.2%, are as follows. First, the general information: 30 cases of 16 males and 14 females, aged 10 to 72 years, suffering from otitis media history of 2 to 30 years. Second, the inspection method: disinfection of the external auditory meatus ear canal ear canal purulent inoculated with L-type medium, set 35 ℃, 5.6% CO 2 incubator incubated for 24 to 48 hours. Colonies observed as gray, small. In the 8 × 10 low magnification under the microscope to pick the “fried egg” or particle-transparent colonies for leather staining, see the different staining, different shapes of rod-shaped giant bodies and sizes of egg-shaped garden ball sphere. For cell wall staining found that cell wall defects as L-shaped bacteria. L-type colonies were picked ordinary agar plate 35 ℃, CO_2 incubator to return ancestor, identification of the strain.