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目的:探讨HTH_1基因治疗帕金森病的可能性。方法:以绿色荧光蛋白(GFP)作为标记,观察HTH_1基因在体外和脑内的表达。结果:构建同时表达GFP和HTH_1双基因的多基因表达载体GC-GFP-P-HTH_1-SN,并转染L-6TG细胞,移植入帕金森病模型大鼠纹状体内,用荧光显微镜、流式细胞仪、免疫组化、Western blotting等方法检测GFP、THT_1在体外和脑内均有表达。结论:本实验为外源基因表达产物的检测提供一个新方法。
Objective: To investigate the possibility of HTH_1 gene therapy for Parkinson’s disease. Methods: Green fluorescent protein (GFP) was used as a marker to observe the expression of HTH_1 gene in vitro and in vivo. Results: The multi-gene expression vector GC-GFP-P-HTH_1-SN that co-expressed GFP and HTH_1 genes was constructed and transfected into L-6TG cells. The cells were transplanted into the striatum of Parkinson’s disease model rats. Fluorescent microscopy Cytometry, immunohistochemistry, Western blotting and other methods to detect GFP, THT_1 expression in vitro and in the brain. Conclusion: This experiment provides a new method for the detection of exogenous gene expression products.