草莓DHAR基因密码子偏好性分析

• 作 者: 刘万达 张丙秀 魏媛媛 王禹 董超 朱延明
• 机 构: 黑龙江省农业科学院园艺分院,东北农业大学园艺学院,东北农业大学生命科学学院,
• 出 处: 北方园艺
• 发表日期: 2014年14期
• 关键词: DHAR 草莓 基因密码子 偏好性 密码子 密码子偏好性 聚类分析 基因表达 作物遗传改良 酵母

以草莓为试材,运用CHIPS、CUSP和CodonW程序分析了草莓DHAR基因的密码子偏好性,并与大肠杆菌和酵母的基因组密码子偏好性及7种植物的DHAR基因密码子偏好性进行了比较,同时基于DHAR基因的密码子使用偏好性进行了系统聚类分析,以期在作物遗传改良中为草莓DHAR基因选择合适的遗传转化受体及进行基因优化提供依据。结果表明:草莓DHAR基因更适合导入苹果等双子叶植物中,若要提高草莓DHAR基因在大肠杆菌或酵母中的表达水平,还需进行密码子的优化。

Using strawberry as test material, the codon preference of strawberry DHAR gene was analyzed by CHIPS, CUSP and CodonW programs and compared with the codon preference of E. coli and yeast and the codon preference of DHAR gene in seven plants , And based on the codon usage preference of DHAR gene, cluster analysis was conducted to provide the basis for selecting suitable genetic transformation receptors and gene optimization for strawberry DHAR gene in crop genetic improvement. The results showed that strawberry DHAR gene was more suitable for introduction into dicots such as apple. To improve the expression of strawberry DHAR gene in E. coli or yeast, codon optimization was also needed.