应用体细胞胚进行苜蓿无性系繁殖和保存的研究

• 作 者: Kenji Okumura 李威
• 出 处: 国外畜牧学(草原与牧草)
• 发表日期: 1997年03期
• 关键词: 体细胞胚 苜蓿品种 无性系 组织培养 高生产性能 外植 愈伤组织 再生力 花粉粒 胚状体

研究应用组织培养技术产生体细胞胚,以达到苜蓿(Medicago sativa L.)无性系繁殖和保存的目的。首先,为建立更有效的培养方法并确定具有较高再生力外植体源,改进培养系统。第二,分析体细胞胚再生和生产性能间的关系,以获得具有较高生产性能的体细胞胚植株。第三,将体细胞无性系的变异效果作为重要性状来研究。第四,用脱水体细胞胚进行无性系保存。最后,就无性系的繁殖和保存在苜蓿育种中的应用进行探讨。

Somatic embryos were generated using tissue culture techniques in order to achieve the purpose of propagation and preservation of alfalfa (Medicago sativa L.) clones. First, to develop more efficient cultivation methods and to identify explants with higher regenerative sources, improve the culture system. Second, the relationship between somatic embryo regeneration and production performance was analyzed to obtain somatic embryo plants with higher productivity. Third, the variation of somaclonal variation as an important trait to study. Fourth, use dehumidified somatic embryos for clonal preservation. Finally, the application of clonal propagation and preservation in alfalfa breeding was discussed.