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用姐妹染色单体交换术(SCE)及~3H-TdRLI％法测定了骨髓相正常的非白血病患者骨髓15例(对照组)及不同病期的成人急性白血病患者骨髓21例(实验组)。结果证明:经72小时培养后,未经治疗的急性白血病患者骨髓细胞仅有3％的中期分裂细胞完成两个分裂周期。而对照组则有40％的中期分裂细胞可完成2～3个分裂周期。用SCE术与~3H-TdRLI％法测定的结果一致,均证明急性白血病细胞的增殖活力明显低于对照组。未治组骨髓细胞为PHA(+)者。即在培养体系中加PHA后可使骨髓细胞总的分裂细胞数(M)或M_2+M_3％增加。此类病人用以S期特异性药物(包括羟基脲和阿糖胞苷)为主的方案疗效较好。骨髓细胞为PHA(-)者,即培养体系中加入PHA后,骨髓细胞M或M_2+M_3％减少。则此类患者用周期非特异性药物(三尖杉酯碱和L-门冬酰胺酶)疗效为佳,以上结果提示白血病细胞生物学特性对治疗效应有重要影响。
Fifteen patients with normal myeloid bone marrow (control group) and 21 patients with adult acute leukemia at different stages (experimental group) were determined by Sister Chromatid Exchanger (SCE) and ~ 3H-TdRLI% methods. The results showed that after 72 hours of culture, only 3% of metaphase cells in untreated acute leukemia cells completed two division cycles. In contrast, 40% of the metaphase dividing cells in the control group were able to complete 2-3 division cycles. The results of SCE and ~ 3H-TdRLI% method were consistent, all showed that the proliferation of acute leukemia cells was significantly lower than the control group. Uncontrolled bone marrow cells PHA (+) were. That is, adding PHA into the culture system can increase the total number of dividing cells (M) or M 2 + M 3% of bone marrow cells. Such patients with S-phase specific drugs (including hydroxyurea and cytarabine) -based program has a better effect. Bone marrow cells PHA (-) who, that PHA added to the culture system, bone marrow M or M 2 + M 3% reduction. Such patients with cycles of non-specific drugs (harringtonine and L-asparaginase) curative effect is better, the above results suggest that the biological characteristics of leukemia cells have a significant impact on the treatment effect.