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目的 医院同期内发现新生儿金黄色葡萄球菌(SA)医院感染,追踪其原因。方法 对患儿、产房、病房环境、洗澡环境、医护人员的手、鼻咽拭子进行病原学调查,并对所分离的SA 测定其MRSA、药敏实验、噬菌体分型、DNA 随机引物扩增(RAPD)来追踪其同源性。结果 采集标本206 份,分离SA55 株,阳性率为38.1% ,6 例患婴均分离出SA,并有2 例合并铜绿假单胞菌感染。洗澡水及洗澡海绵取标本12 份,均培养出SA。噬菌体分型,患婴组及洗澡组均为Ⅲ型,其余环境SA 多为Ⅰ型。结论 患婴SA 和洗澡海绵SA 为同一聚类群属同源性。SA特异基因PCR 诊断与耐药基因测定对追踪同源性意义不大
The hospital of destination was found to have neonatal S. aureus (SA) nosocomial infections in the same period and to track the cause. Methods The etiological investigation of children, maternity ward, ward environment, bathing environment, medical staff’s hand and nasopharyngeal swab was performed. The isolated SA was assayed for MRSA, drug susceptibility test, phage typing and DNA random primer amplification (RAPD) to track its homology. Results A total of 206 samples were collected and SA55 strains were isolated. The positive rate was 38.1%. SA was isolated from 6 infants and 2 cases were infected with Pseudomonas aeruginosa. Take 12 samples of bath water and bath sponge, cultivate SA. Phage typing, suffering from infants group and bathing group are type Ⅲ, the rest of the environment SA mostly type Ⅰ. Conclusion The results showed that both SA and SH sponge were homologous to the same cluster. The diagnosis of SA-specific genes and the determination of drug resistance genes have little significance for tracking homology