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为探讨巨细胞病毒(CMV)UL44(P52)、UL32(P150)特异性免疫球蛋白M(抗P52-IgM、抗P150-IgM)与CMV肝炎的临床关系。运用免疫印迹技术(IB)对26例CMV肝炎患儿外周血特异性抗P52-IgM、抗P150-IgM进行检测。结果:26例患儿中抗P52-IgM阳性率为100％,抗P150-IgM阳性率为61.5％;先天感染抗P150-IgM阳性率明显高于围生期感染(P<0.01);围生期抗P150-IgM阳性患儿其白细胞计数、直接胆红素值、γ-谷氨酰转肽酶值、锁骨中线肋下肝肿大值均高于抗P150-IgM阴性患儿(P分别<0.01、0.01、0.01、0.05)。提示抗P52-IgM和抗P150-IgM均阳性与疾病严重性有一定关系,抗P52-IgM阳性在确诊CMV肝炎中有很高的敏感性。
To investigate the clinical relationship between cytomegalovirus (CMV) UL44 (P52), UL32 (P150) -specific immunoglobulin M (anti-P52-IgM, anti-P150-IgM) and CMV hepatitis. The peripheral blood specific anti-P52-IgM and anti-P150-IgM in 26 patients with CMV hepatitis were detected by Western blotting (IB). Results: The positive rate of anti-P52-IgM was 100% and the anti-P150-IgM was 61.5% in 26 cases. The positive rate of anti-P150-IgM in congenital infection was significantly higher than that in perinatal infection (P <0.01) The white blood cell count, direct bilirubin, γ-glutamyl transpeptidase, and subclavian hepatomegaly hepatomegaly were significantly higher in patients with positive anti-P150-IgM than in children with anti-P150-IgM negative (P < 0.01, 0.01, 0.05). It is suggested that the positive of anti-P52-IgM and anti-P150-IgM are related to the severity of the disease. The positive anti-P52-IgM is highly sensitive in diagnosing CMV hepatitis.