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本文利用同步辐射产生的Ck软X线（E=0．28KeV），以60Co-γ射线为对照，研究了Ck软x线对质粒PBK－CMVDNALacZ基因片段的诱变作用及对其转化活性的影响，并对Ck软X线所诱导的9个LacZ-突变体LacZ基因片段进行了序列测定。结果显示：同60Co-γ射线相比，Ck软X线具有高效的生物效应，表现为较高的诱导突变率与转化失活率（1一存活率）。Ck软X钱诱导LacZ基因突变的主要特点有：碱基变异类型多，并以碱基置换为主；碱基变异的发生频率较高；突变在位点916、969、996、1083处发生的次数较多，它们可能与Ck软X线的突变热点有关。Ck软X线的分子突变谱的研究将有助于从分子水平揭示其生物效应的机制，从本质上看：Ck软X线的生物效应主要是由软X线与受照物质之间相互作用特点决定的。
In this paper, the Ck soft X-ray (E = 0.28KeV) produced by synchrotron radiation was used to study the mutagenesis effect of Ck soft x-ray on the plasmid PBK-CMVDNALacZ gene fragment and its effect on the transformation activity , And nine LacZ-LacZ gene fragments induced by Ck soft X-ray were sequenced. The results showed that compared with 60Co-γ-ray, Ck soft X-ray has high biological effect, showing high induction mutation rate and transformation inactivation rate (1-survival rate). Ck soft X money LacZ gene mutations induced by the main features are: more than the type of base mutation, and base-based replacement; base mutation is higher frequency of mutations in the site 916,969,996,1083 place More times, they may be related to Ck soft X-ray mutation hot spots. Ck soft X-ray molecular mutation spectrum research will help to reveal its biological effects at the molecular level mechanism, in essence, the biological effect of Ck soft X-ray is mainly caused by the interaction between the soft X-ray and the irradiated material Characteristics of the decision.