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对疟原虫子孢子期的研究,由于以感染蚊分离和纯化子孢子较困难而受到影响。本文介绍一种用锥形试管和低速离心分离蚊体内子孢子的快速、简单方法。取一支0.5ml的锥形管,用热的20号皮下注射针在其底部穿一小孔,塞以小块的玻璃棉,放入另1支1.5ml的锥形试管中。将麻醉后的感染蚊放在玻璃板上摇动,用含有0.2%牛血清白蛋白液的M_(199)将蚊子湿
Studies on the sporozoite stage of Plasmodium have been affected by the difficulty in isolating and purifying sporozoites from infected mosquitoes. This article presents a quick and easy way to separate sporozoites from mosquitoes using conical tubes and low-speed centrifugation. Take a 0.5ml conical tube, use a hot 20 hypodermic needle to wear a small hole in the bottom, plug the small pieces of glass wool into another 1.5ml conical tube. The anesthetized infected mosquito was placed on a glass plate and shaken. The mosquito was wet with M_ (199) containing 0.2% bovine serum albumin