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目的:探讨创伤应激状态下小鼠免疫功能变化及黄芪多糖(APS)对应激状态下机体免疫功能影响。方法:建立小鼠截肢应激模型,将50只Balb/c小鼠随机分为5组: 正常对照组(A);应激对照组(B);应激+ 黄芪多糖高(C)、中(D)、低(E)剂量组,每组10只。创伤后72小时称小鼠胸腺、脾脏湿重,用原位杂交检测胸腺、脾脏淋巴细胞中NF-κB mRNA、IL-10mRNA表达,计算机图像分析系统定量。结果:与正常对照组(A)比较,创伤后72小时应激对照组(B)小鼠胸腺、脾脏重量指数明显下降(P<0.01=;胸腺、脾脏淋巴细胞NF-κBmRNA、IL-10mRNA表达水平均明显升高(P<0.01);与创伤应激对照组(B)比较,应激+ 黄芪多糖高(C)、中(D)、低(E)剂量组小鼠胸腺、脾脏指数有不同程度的升高,胸腺、脾脏淋巴细胞中NF-κB mRNA、IL-10mRNA的表达受抑(P<0.01)。与正常对照组(A)比较,应激+ APS高剂量组(C)小鼠胸腺、脾脏指数;胸腺、脾脏组织中NF-κB mRNA、IL-10mRNA的表达无显著性差异(P>0.01>0.05)。结论:创伤后小鼠免疫功能受抑;黄芪多糖体内应用可有效恢复其免疫功能。
OBJECTIVE: To investigate the changes of immune function and the effects of astragalus polysaccharides (APS) on immune function under stress in mice. METHODS: A mouse amputation stress model was established and 50 Balb/c mice were randomly divided into 5 groups: normal control group (A); stress control group (B); stress + astragalus polysaccharide (C), medium (D) Low (E) dose groups of 10 per group. The weight of thymus and spleen of mice was weighed 72 hours after trauma. The expression of NF-κB mRNA and IL-10 mRNA in lymphocytes of thymus and spleen were detected by in situ hybridization and quantified by computer image analysis system. RESULTS: Compared with the control group (A), the weight index of thymus and spleen in the stress control group (B) was significantly decreased at 72 hours after trauma (P<0.01=; thymus and spleen lymphocyte NF-κB mRNA, IL-10 mRNA expression The levels were significantly higher (P<0.01). Compared with the traumatic stress control group (B), the thymus and spleen indexes of the mice with high (C), medium (D), and low (E) doses of stress + Astragalus polysaccharides had With different degrees of increase, the expression of NF-κB mRNA and IL-10 mRNA in lymphocytes of thymus and spleen was inhibited (P<0.01), compared with the normal control group (A), the stress + APS high dose group (C) was small There was no significant difference in the expression of NF-κB mRNA and IL-10 mRNA between the thymus and spleen tissues (P>0.01>0.05).Conclusions: The immune function of mice after trauma is inhibited, and the application of astragalus polysaccharides in vivo is effective. Restore its immune function.