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目的:观察穿心莲内酯对人结肠癌SW1116细胞生长及肿瘤细胞凋亡的影响,探讨其可能的机制。方法:用不同浓度的穿心莲内酯处理SW1116细胞,MTT检测穿心莲内酯对SW1116细胞生长增殖的抑制作用;流式细胞术(FCM)检测细胞凋亡率;比色法测定Caspase-3酶活性;Western blot法检测bax、Bcl-2的蛋白表达。结果:穿心莲内酯能够剂量依赖型的抑制人结肠癌SW1116细胞的增殖,并诱导凋亡;穿心莲内酯与SW1116细胞作用48小时后Caspase-3酶活性显著增强;同时,穿心莲内酯处理SW1116细胞后,Bax蛋白表达增强,Bcl-2蛋白表达下调。结论:穿心莲内酯可抑制人结肠癌SW1116的增殖,诱导其凋亡,机制可能与调节Caspase-3、Bcl-2和Bax表达水平有关。
Objective: To observe the effects of andrographolide on the growth of human colon cancer cell line SW1116 and the apoptosis of tumor cells, and to explore its possible mechanism. Methods: SW1116 cells were treated with different concentrations of andrographolide. The inhibitory effect of andrographolide on the growth and proliferation of SW1116 cells was detected by MTT assay. The apoptosis rate was detected by flow cytometry (FCM). The activity of Caspase-3 was assayed by colorimetric assay. Western blot was used to detect the protein expression of bax and Bcl-2. Results: Andrographolide could inhibit proliferation and induce apoptosis of human colon cancer SW1116 cells in a dose-dependent manner. Caspase-3 activity was significantly increased after andrographolide and SW1116 cells were treated for 48 hours. Meanwhile, andrographolide treatment of SW1116 cells After Bax protein expression increased, Bcl-2 protein expression down. Conclusion: Andrographolide can inhibit the proliferation and induce apoptosis of human colon cancer SW1116 cells. The mechanism may be related to the regulation of Caspase-3, Bcl-2 and Bax expression.