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目的观察戊四氮点燃致癎发育期大鼠模型行为改变和脑内突触重建情况及天麻素对其影响。方法将50只21日龄Wistar发育期大鼠(50~70g)随机分为5组:对照组、戊四氮组、天麻素大剂量组、天麻素小剂量组及丙戊酸钠组,每组10只。根据Racine分级法,观察大鼠行为学改变,记录大鼠癎性发作级别和次数。在点燃4周后常规心脏灌注、固定及取脑组织,采用免疫组织化学方法检测各组大鼠组织切片突触体素(P38)的表达。结果1.戊四氮点燃4周后,各实验组大鼠癎性发作级别比较具有统计学意义(χ2=35.83P<0.01),戊四氮组发作级别最高(平均秩为44.60),其次是天麻素小剂量组(平均秩为34.90)、天麻素大剂量组(平均秩为21.45)、丙戊酸钠组(平均秩为19.55)。2.戊四氮点燃4周后,各组实验大鼠颞叶皮质及海马区均有P38表达,戊四氮组P38表达量较其他组多,有统计学差异(Pa<0.01),天麻素大剂量组表达量小于天麻素小剂量组(q=2.705,2.351Pa<0.05),天麻素大剂量组P38表达量与丙戊酸钠组比较均无统计学意义(q=1.194,1.031Pa>0.05)。结论戊四氮反复点燃可导致发育期大鼠颞叶和海马区苔藓纤维发芽和突触重建的形成;天麻素具有明显抗癫癎作用,可通过降低P38的表达,抑制突触重建的形成,间接控制癫癎发作。
Objective To observe the behavioral changes of rat model induced by pentylenetetrazole (PTZ) and the synaptic reconstruction in brain and the effect of gastrodin on it. Methods Fifty 50-day-old Wistar rats (50-70g) were randomly divided into five groups: control group, pentylenetetrazol group, gastrodine high-dose group, gastrodin low-dose group and sodium valproate group Group of 10. According to the Racine grading method, the behavioral changes of rats were observed and the severity and frequency of malaria seizures were recorded. Normal heart perfusion, fixation and brain tissue were taken after 4 weeks of ignition. The expression of synaptophysin (P38) in each group was detected by immunohistochemical method. Results 1. After 4 weeks of pentylenetetrazole ignition, the seizure severity of rats in each experimental group was statistically significant (χ2 = 35.83 P <0.01), and pentylenetetrazole was the highest (average rank 44.60), followed by Gastrodin low dose group (average rank was 34.90), gastrodin high dose group (average rank was 21.45), sodium valproate group (average rank was 19.55). After 4 weeks of pentylenetetrazole lighting, the expression of P38 in temporal lobe cortex and hippocampus of experimental rats in each group was higher than that of other groups (P <0.01) The expression of P38 in the high-dose group was lower than that in the low-dose gastrodin group (q = 2.705, 2.351Pa <0.05). There was no significant difference in the expression of P38 between the high-dose gastrodin group and the sodium valproate group (q = 1.194,1.031Pa> 0.05). Conclusions Pentylenetetrazole can cause the formation of mossy fiber sprouts and synaptic remodeling in the temporal lobe and hippocampus of rats during development. Gastrodin has obvious antiepileptic effect and inhibits the formation of synaptic remodeling by decreasing the expression of P38, Indirect control of epileptic seizures.