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目的探讨二氧化硫(SO2)对低氧性肺动脉高压大鼠肺动脉胶原堆积的影响。方法将雄性Wistar大鼠34只分为对照组(8只)、低氧组(8只)、低氧加SO2组(腹腔注射亚硫酸钠/亚硫酸氢钠混合液0.5mL/d)(9只)和低氧加天冬氨酸异羟肟酸(HDX)组[腹腔注射HDX3.7mg/(kg·d)](9只)。对低氧组、低氧加SO2组和低氧加HDX组大鼠进行低氧处理21d,氧体积分数为(100±5)mL/L,低氧处理6h/d。采用羟脯氨酸水平测定及天狼猩红染色法分析低氧性肺动脉高压大鼠肺组织及肺动脉胶原的变化。采用SPSS13.0软件进行统计学分析。结果4组大鼠肺组织羟脯氨酸水平有显著性差异(F=12.363 Pa<0.01)。与对照组比较,低氧组大鼠羟脯氨酸水平升高34.53%(P<0.01),随着低氧性肺动脉高压的形成,大鼠肺动脉中羟脯氨酸水平升高。与低氧组比较,低氧加SO2组大鼠羟脯氨酸水平降低17.58%(P<0.05),低氧加HDX组大鼠羟脯氨酸水平升高21.30%(P<0.05)。天狼猩红偏振光法分析显示,与对照组比较,低氧组大鼠血管壁胶原纤维明显增加;与低氧组比较,低氧加SO2组大鼠血管壁Ⅰ型胶原较少,呈红色或黄色粗纤维状,与Ⅲ型胶原伴行,低氧加HDX组大鼠血管壁胶原明显增加。结论SO2能显著降低低氧大鼠的肺动脉胶原堆积。
Objective To investigate the effect of sulfur dioxide (SO2) on pulmonary artery collagen accumulation in hypoxic pulmonary hypertensive rats. Methods Thirty-four male Wistar rats were divided into control group (n = 8), hypoxia group (n = 8), hypoxia plus SO2 group (intraperitoneal injection of sodium sulfite / sodium bisulfite mixture 0.5 mL / d) And hypoxia plus aspartic hydroxamic acid (HDX) group [HDX3.7mg / (kg · d)] (n = 9). The hypoxia group, hypoxia plus SO2 group and hypoxia plus HDX group were treated with hypoxia for 21 days, and the oxygen volume fraction was (100 ± 5) mL / L and hypoxia treatment was 6h / d. Hydroxyproline levels and Sirius red staining were used to analyze the changes of collagen in pulmonary tissue and pulmonary artery in hypoxic pulmonary hypertensive rats. SPSS13.0 software was used for statistical analysis. Results There was a significant difference in hydroxyproline level between the four groups (F = 12.363 Pa <0.01). Compared with the control group, the level of hydroxyproline in hypoxia group increased by 34.53% (P <0.01). With the formation of hypoxic pulmonary hypertension, the level of hydroxyproline in pulmonary artery of rats increased. Compared with the hypoxia group, the hydroxyproline level decreased by 17.58% (P <0.05) in the hypoxia plus SO2 group, and the hydroxyproline level increased by 21.30% (P <0.05) in the hypoxia plus HDX group. Sirius red polarized light analysis showed that, compared with the control group, hypoxic group of vascular wall collagen fibers increased significantly; compared with the hypoxia group, hypoxia plus SO2 group of vascular wall less collagen type I, red or Yellow crude fiber, accompanied by type Ⅲ collagen, hypoxia plus HDX group significantly increased vascular wall collagen. Conclusion SO2 can significantly reduce pulmonary artery collagen accumulation in hypoxic rats.