论文部分内容阅读
目的:探索一种简便的动态检测胰腺腺泡细胞内钙离子浓度的方法。方法:无菌采集大鼠胰腺，去除脂肪和淋巴组织，37 ℃预热的胶原酶消化胰腺组织，分离大鼠胰腺腺泡细胞。加入钙离子荧光探针Fluo-3/AM负载腺泡细胞，流式细胞仪动态监测腺泡细胞内钙离子浓度变化。结果:对照组腺泡细胞处于静息状态，钙离子荧光强度稳定，8只大鼠胰腺腺泡细胞内钙离子浓度随时间变化趋势一致，平均荧光强度值为307.75±36.45(n n＝8)；刺激组加入牛磺胆酸钠后腺泡细胞钙离子荧光强度升高，第27.76秒达峰值，检测结果稳定。n 结论:流式细胞仪为腺泡细胞钙离子浓度和细胞功能研究、及后续急性胰腺炎发病机制的研究提供另一简便易行的方法。“,”Objective:To explore a convenient method for dynamic monitoring of cytosolic calcium concentration of rat pancreatic acinar cells.Methods:The rat pancreas was collected aseptically and the fat and lymph tissues were removed. Then the rat pancreatic acinar cells were isolated by collagenase preheated at 37 ℃. The pancreatic acinar cells were incubated with Fluo-3/AM. The dynamic changes of calcium concentration were real-time monitored by flow cytometry.Results:The acinar cells of the control group were in a static status, there was no change in the cell fluorescence intensity over time. The calcium concentration in the acinar cells of the 8 rats in this experiment showed a consistent trend over time, with an average fluorescence intensity value of 307.75±36.45 (n n=8). The cell fluorescence intensity increased and reached the peak value in 27.76 seconds when adding the irritant sodium taurocholate to the cells compared with that before the stimulation.n Conclusion:Flow cytometry provides another convenient method for the study of calcium cocentration and cell function of acinar cells and the subsequent study of the pathogenesis of acute pancreatitis.