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合成了一种新的席夫碱配体2,4-二羟基苯乙酮缩三羟基甲基氨基甲烷。该配体与铽(Ⅲ)的配合物的荧光强度在磷酸钠或六次甲基四胺的存在下可增强18或26倍。有机溶剂CH_3CN,DMF,丙酮,THF,DMSO,CH_3OH,C_2H_5OH的添加可使其荧光强度进一步增加3～19倍。其中,用CH_3CN作溶剂时荧光增加倍数最大,Tb-HL-HMTM体系荧光最强。pH=6.0时,激发和发射波长分别是355和545nm。在理想情况下,荧光强度与Tb~(3+)离子浓度成正比。对于HL-Na_3PO_4-CH_3CN体系,线性范围2.3×10~(-10)～5.8×10~(-5) mol/L,检测限2.0×10~(-10) mol/L,对于HL-HMTM-CH_3CN体系,线性范围9.8x10_(-11)～7.2×10~(-5) mol/L,检测限9.0×10~(-11) mol/L。基于此对Tb_(3+)进行定量测定,该分析方法简单、快速、灵敏、干扰小。同时对荧光增强的机理进行了分析和讨论。
A new Schiff base ligand, 2,4-dihydroxyacetophenone, was synthesized. The fluorescence intensity of the complex with terbium (III) can be enhanced by 18- or 26-fold in the presence of sodium phosphate or hexamethylenetetramine. The addition of CH_3CN, DMF, acetone, THF, DMSO, CH_3OH and C_2H_5OH can increase the fluorescence intensity by 3 ~ 19 times. Among them, when using CH_3CN as the solvent, the fluorescence multiplication factor is the largest, and Tb-HL-HMTM system has the strongest fluorescence. At pH = 6.0, the excitation and emission wavelengths are 355 and 545 nm, respectively. Under ideal conditions, the fluorescence intensity is proportional to the Tb 3+ ion concentration. For the HL-Na 3 PO 4-CH 3 CN system, the linear range was from 2.3 × 10 -10 to 5.8 × 10 -5 mol / L and the detection limit was 2.0 × 10 -10 mol / L. For HL-HMTM- CH_3CN system. The linear range was 9.8 × 10 ~ (- 11) ~ 7.2 × 10 ~ (-5) mol / L with the detection limit of 9.0 × 10 ~ (-11) mol / L. Based on this determination of Tb_ (3+), this method is simple, rapid, sensitive and has little interference. At the same time, the mechanism of fluorescence enhancement was analyzed and discussed.