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为进一步明确细胞色素P450 CYP6B7、细胞色素b5(cytochrome b5,Cyt-b5)和细胞色素P450还原酶(cytochrome P450 reductase,CPR)在棉铃虫Helicoverpa armigera(Hübner)对拟除虫菊酯类药剂抗性中的作用,通过RNA干扰技术沉默了抗氰戊菊酯棉铃虫中3种组分的基因,并测定基因沉默后高效氯氰菊酯对棉铃虫的毒力变化。结果显示,经单个CYP6B7或CYP6B7与CPR、Cyt-b5基因的双链RNA注射处理后12~48 h,棉铃虫幼虫死亡率在19.4%~92.5%之间,明显高于空白对照处理的10.47%~62.87%;高效氯氰菊酯对基因沉默后幼虫的LD50值随着时间的延长而逐渐下降,12~48 h的LD50值在0.97~2.97μg/头之间,而空白对照处理为2.27~3.57μg/头。研究表明,细胞色素P450 CYP6B7,或CYP6B7与CPR、Cyt-b5基因的沉默,可提高高效氯氰菊酯对抗性棉铃虫的毒力,进一步证明了CYP6B7、CPR和Cyt-b5在棉铃虫对菊酯类药剂抗性中起着重要作用。
In order to further clarify the resistance of pyrethrins to Helicoverpa armigera (Hübner) to cytochrome P450 CYP6B7, cytochrome b5 (Cyt-b5) and cytochrome P450 reductase (CPR) RNA interference technique was used to silence the three components of fenvalerate resistance in Helicoverpa armigera and to determine the change of beta-cypermethrin toxicity to Helicoverpa armigera after gene silencing. The results showed that the mortality of H. armigera larvae was between 19.4% and 92.5% after 12-48 h injection of double-stranded RNA of single CYP6B7 or CYP6B7 and CPR and Cyt-b5 genes, which was significantly higher than that of the control (10.47% ~ 62.87%, respectively. The LD50 value of beta-cypermethrin after gene silencing gradually decreased with time increasing. The LD50 value ranged from 0.97 to 2.97 μg / head at 12-48 h, while it was 2.27-3.57 μg / head. The results showed that cytochrome P450 CYP6B7, or CYP6B7 and CPR, Cyt-b5 gene silencing, can improve the beta-cypermethrin-resistant cotton bollworm virulence further CYP6B7, CPR and Cyt-b5 in cotton bollworm to pyrethroid agents Resistance plays an important role.