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以杉木不同基因型的未成熟胚为起始外植体,研究不同冷藏时间、不同接种方式、不同基因型和不同激素浓度组合对胚性愈伤组织诱导率的影响。结果表明,冷藏显著影响种子的质量,随着冷藏时间延长,坏死种子比例显著增加,冷藏20 d时坏死种子比例达54.5%;冷藏对胚性愈伤组织诱导率无显著影响。接种方式对胚性愈伤组织诱导具有显著影响,接种方式二(剥去种皮,切开小口)的诱导效果最好,方式一(种子切开小口)和方式三(剥出合子胚)不利于胚性愈伤组织诱导。不同基因型在同一激素组合条件下的胚性愈伤组织诱导率存在显著差异,同一基因型在不同激素组合下的胚性愈伤组织诱导率也存在显著差异。总体看来,6-BA对胚性愈伤组织诱导率的影响最大,其最优浓度为1.0 mg/L;其次为2,4-D和ABA,其较优浓度均为2.0 mg/L;KT较优浓度为1.0 mg/L。
In this experiment, immature embryos with different genotypes of Cunninghamia lanceolata were used as explants to study the effects of different cold storage time, different inoculation methods, different genotypes and different hormone concentrations on embryogenic callus induction rate. The results showed that cold storage significantly affected the quality of seeds. With the extension of refrigeration time, the proportion of necrotic seeds increased significantly, and the proportion of dead seeds reached 54.5% after 20 days of cold storage. Cold storage had no significant effect on the induction rate of embryogenic callus. Inoculation method had significant effect on embryogenic callus induction. The best induction effect was obtained by inoculation method two (peeling the seed coat, cutting the small opening), the first method (seed cutting small mouth) and the third method (peeling zygotic embryo) Conducive to embryogenic callus induction. There were significant differences in embryogenic callus induction rates among different genotypes under the same hormone combination. There were also significant differences in embryogenic callus induction rates among different genotypes under the same genotype. Overall, 6-BA had the greatest effect on the induction rate of embryogenic callus, with the optimal concentration being 1.0 mg / L, followed by 2,4-D and ABA, with the optimal concentration of 2.0 mg / L. The optimal concentration of KT was 1.0 mg / L.