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多形核白细胞(PML)产生氧自由基,降低精子活动性及与卵细胞融合的能力;抗氧化剂可以逆转氧自由基的损伤效应,因而可能保护精子免受损伤。 采多供体混合精液,Percoll梯度离心后用抗CD_(45)磁珠处理,去除白细胞,制备为纯化精子。由H:Stopaque或Polyprep梯度离心,分离外周血PML,用离心后精浆按不同密度混悬,并加PML活化剂PMA100mM。纯化精子重新混悬于含PML的精浆,使精子密度为20×10~6/ml,37℃培养半小时。由
Polymorphonuclear leukocytes (PML) produce oxygen free radicals, reducing sperm motility and ability to fuse with egg cells; antioxidants can reverse the damaging effects of oxygen free radicals and thus may protect sperm from injury. Multi-donor mixture of semen, Percoll gradient centrifugation after treatment with anti-CD_ (45) beads, removal of white blood cells, prepared for the purification of sperm. Peripheral blood PML was separated by gradient centrifugation from H: Stopaque or Polyprep, suspended with different densities after centrifugation and supplemented with PMA activator PMA100mM. Purified sperm resuspended in PML-containing seminal plasma so that the sperm density of 20 × 10 ~ 6 / ml, 37 ℃ for half an hour. by