论文部分内容阅读
The effects of ethephon and abscisic acid(ABA) application on ripening-related genes of pre-harvest ‘Kohi’ kiwifruit(Actinidia chinensis) were studied to clarify the possibility of the fruit ripening on the vine. The fruits were treated on the vine at mature stage with 250 μL·L~(-1)ethephon or 100 μmol·L~(-1)ABA, and the expression levels of chlorophyll synthase(Ac CLS), chlorophyllase1(Ac CLH1), polygalacturonase(Ac PG), expansin(Ac EXP),β-amylase(Acβ-AM), sucrose synthase(Ac SUSY), MADS-box SEPALLATA4/RIPENING INHIBITOR-like(Ac SEP4/RIN) and FRUITFUL-like(Ac TDR4/FUL) genes were analyzed. The expression levels of Ac PG, Ac EXP, Acβ-AM, and Ac SUSY increased in the ethephon-treated fruit, but those of Ac CLH1 at 6and 9 days after treatment and Ac CLS decreased. Moreover, the expression levels of Ac SEP4/RIN and Ac TDR4/FUL, the latter of which is associated with ethylene biosynthesis, were higher in the ethephon-treated fruit. The expression level of each gene in ABA-treated fruit was not significantly different from that of the untreated control. The results suggest that ethephon application increases the expression levels of Ac PG,Ac EXP, Acβ-AM, Ac SUSY, Ac SEP4/RIN, and Ac TDR4/FUL in ‘Kohi’ kiwifruit on the vine.
The effects of ethephon and abscisic acid (ABA) application on ripening-related genes of pre-harvest ’Kohi’ kiwifruit (Actinidia chinensis) were studied to clarify the possibility of the fruit ripening on the vine. The fruits were treated on the vine at mature stage with 250 μL·L -1 ethephon or 100 μmol·L -1 ABA, and the expression levels of chlorophyll synthase (Ac CLS), chlorophyllase 1 (Ac CLH1), polygalacturonase (Ac PG), expansin (Ac EXP), β-amylase (Acβ-AM), sucrose synthase (Ac SUSY), MADS-box SEPALLATA4 / RIPENING INHIBITOR-like (Ac SEP4 / RIN) and FRUITFUL- like (Ac TDR4 / FUL) genes were analyzed. The expression levels of Ac PG, Ac EXP, Acβ-AM, and Ac SUSY increased in the ethephon-treated fruit, but those of Ac CLH1 at 6 and 9 days after treatment and Ac CLS decreased. Furthermore, the expression levels of Ac SEP4 / RIN and Ac TDR4 / FUL, the latter of which is associated with ethylene biosynthesis, were higher in the ethephon-treated fruit. The expression level of each gene in ABA-treated The results suggest that from ethephon application increases the expression levels of Ac PG, Ac EXP, Acβ-AM, Ac SUSY, Ac SEP4 / RIN, and Ac TDR4 / FUL in ’Kohi’ kiwifruit on the vine