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建立丙型肝炎病毒(HCV)的细胞模型及动物模型,对于深入认识HCV病原学特点、复制及发病机制,筛选有效药物及疫苗等极为重要。近年来,国内外学者作了大量探讨,概括如下。 1 HCV细胞模型的研究 1.1 HCV感染类细胞模型 1.1.1 以肝细胞为宿主的HCV感染细胞模型 众所周知,人肝细胞是HCV感染的天然宿主细胞,以成人肝细胞作为感染HCV的细胞模型应有更好的代表性。Ito等直接将HCV阳性血清与丙肝患者肝活检分离出来的肝细胞共培养,发现肝细胞内和培养上清液中可检出大量HCV RNA,用
The establishment of a cell model and an animal model of Hepatitis C virus (HCV) is of great importance for understanding the etiological characteristics, replication and pathogenesis of HCV, screening effective drugs and vaccines, and so on. In recent years, domestic and foreign scholars made a lot of discussion, summarized as follows. 1 HCV CELL MODELS 1.1 HCV-infected CELL MODELS 1.1.1 HCV-infected Cell Models with Hepatocytes as Host It is well-known that human hepatocytes are natural host cells for HCV infection and that adult hepatocytes should be used as a cell model for HCV infection Better representation. Ito et al directly co-cultured hepatocytes isolated from HCV-positive sera and liver biopsies of patients with hepatitis C and found that a large amount of HCV RNA was detected in hepatocytes and in culture supernatants