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目的探讨亚低温和硫酸镁对缺氧缺血脑损伤（ＨＩＢＤ）的保护作用。方法７日龄ＳＤ大鼠２８２只（体重：１４ｇ±２．３ｇ）右颈总动脉永久性结扎加８％氧气２小时。将此模型动物随机分为亚低温干预组（ｈｙ６０只）；硫酸镁（ＭｇＳＯ４）干预组（Ｍｇ６２只）；联合干预组（ＨＭ６０只）；室温恢复组（ＲｏＴ６０只）。５组动物于实验后２４小时、４８小时、７２小时测血浆Ｓ１００蛋白（Ｓ１００）和肌酸激酶脑型同功酶（ＣＫＢＢ）水平。实验２周后以脑组织病理切片的右侧脑损伤分数（ＢＩＳ）和右侧海马细胞死亡率（ＨＮＮＰ）判定疗效。结果ＲｏＴ、Ｍｇ、Ｈｙ和ＨＭ４组动物干预期间肛温（ＲＴ）平均为３６．６°Ｃ，３６．７°Ｃ，３２．５°Ｃ和３２．４°Ｃ，脑温（ＢＴ）低于ＲＴ０．３°Ｃ～０．６°Ｃ（γ＝０．９９，Ｐ＜０．０１）。ＲｏＴ组血浆Ｓ１００、ＣＫＢＢ于２４小时～４８小时达峰值（分别为１．２１μｇ／Ｌ～１．２４μｇ／Ｌ和５０．３ＩＵ／Ｌ～５７．２ＩＵ／Ｌ），是正常的２～３倍，３个干预组血浆Ｓ１００和ＣＫＢＢ水平明显低于ＲｏＴ组（Ｐ＜０．０１）。病理结果显示：ＲｏＴ、Ｍｇ、Ｈｙ和ＨＭ四组ＢＩＳ分别为３．２、１．８、０．３和１．２；Ｈ?
Objective To investigate the protective effects of mild hypothermia and magnesium sulfate on hypoxic-ischemic brain damage (HIBD). Methods 282 SD rats aged 7 days (body weight: 14g ± 2.3g) were subjected to permanent ligation of the right common carotid artery with 8% oxygen for 2 hours. The model animals were randomly divided into hypothermia intervention group (hy60 only); MgSO4 intervention group (Mg62 only); combined intervention group (HM60 only); room temperature recovery group (RoT60 only). Five groups of animals in the experiment 24 hours, 48 hours, 72 hours measured plasma S 100 protein (S 100) and creatine kinase brain isozyme (CK BB) levels. Two weeks after the experiment, the right brain injury score (BIS) and the right hippocampal cell death rate (HNNP) were used to determine the curative effect. Results The average rectal temperature (RT) of RoT, Mg, Hy and HM4 animals were 36.6 ° C, 36.7 ° C, 32.5 ° C and 32.4 ° C, and the mean cerebral temperatures (BT) RT0.3 ° C ~ 0.6 ° C (γ = 0.99, P <0.01). Serum S100 and CKBB in RoT group reached peak at 24 hours to 48 hours (1.21μg / L ~ 1.24μg / L and 50.3IU / L ~ 57.2IU / L, respectively) ~ 3 times, the levels of plasma S100 and CKBB in three intervention groups were significantly lower than those in RoT group (P <0.01). Pathological results showed that the BIS of RoT, Mg, Hy and HM four groups were 3.2, 1.8, 0.3 and 1.2 respectively; H?