C型肉毒梭菌毒素对小白鼠LD_(50)测定及现场灭鼠效果观察

• 作 者: 李述杨 张增炬 吴锡进 陈小彬 詹绍琛
• 机 构: 福建省地方病防治研究所,尤溪县爱卫会办公室,莆田市卫生防疫站
• 出 处: 中国媒介生物学及控制杂志
• 发表日期: 1998年01期
• 关键词: 灭鼠效果 LD 肉毒梭菌 半数致死量 毒力 灌胃法 可信限 鼠密度 现场试验 制剂组

目的：观察Ｃ型肉毒梭菌毒素对小白鼠的ＬＤ５０及现场灭鼠效果。方法：毒力测定采用定量药液灌胃法；用粉迹法调查鼠密度，计算灭鼠率。结果：该药在２５℃、３０℃、３５℃放置４８小时，对小白鼠的ＬＤ５０分别为００８９１±００４７３、０１３６３±００５９７和０１７１２±００６５２ｍｌ／ｋｇ；现场试验，昼最高气温２０～２５℃，夜最低气温１５～１８℃，３个试点村的灭鼠率分别为９１０８％、８９４１％、８８０４％。结论：Ｃ型肉毒梭菌毒素的毒力甚强，适宜在我国南方地区推广使用

Objective: To observe the LD50 of C. botulinum toxin C and its anti-rat effect in mice. Methods: Toxicity was determined by the method of gavage; gavage method was used to investigate the density of mice and the rodent rate was calculated. Results: The LD50 of the drug was 08991 ± 00473, 01363 ± 0057 and 01712 ± 00652ml / kg for 48 hours at 25 ℃, 30 ℃ and 35 ℃, respectively. Field experiments showed that the daily maximum temperature was 20-25 ℃ and night minimum temperature was 15-18 ℃. The rodent control rates of the three pilot villages were 9108%, 8941% and 8804% respectively. Conclusion: Clostridium botulinum type C toxin is very virulent and suitable for popularization in southern China