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目的：研究４种药物对体外成纤维细胞培养的增殖抑制作用及有效浓度。方法：对传代培养的成纤维细胞经一定浓度的柔红霉素、阿霉素、地塞米松、５－ＦＵ培养４８ｈ后，观察细胞生长情况，并分别求得对兔皮肤成纤维细胞的半数抑制浓度（ＩＤ５０）。结果：柔红霉素、阿霉素对体外成纤维细胞的增殖均有显著抑制作用，呈浓度依赖性改变；地塞米松和５－ＦＵ的抑制作用相对较弱，但地塞米松分别与其他３种药物的半数抑制浓度合用后的效果，比单用上述４种药物的抑制作用明显增强。结论：柔红霉素、阿霉素能有效抑制体外兔成纤维细胞，并提示与地塞米松合用可能成为有价值的防治增殖性玻璃体视网膜病变（ＰＶＲ）的用药方式
Objective: To study the inhibitory effect and effective concentration of four drugs on the proliferation of fibroblasts cultured in vitro. Methods: The cultured fibroblasts were cultured with certain concentration of daunorubicin, doxorubicin, dexamethasone and 5-FU for 48h, and the growth of fibroblasts were observed. Half of the fibroblasts were obtained from the skin fibroblasts Inhibitory concentration (ID50). Results: Daunorubicin and doxorubicin significantly inhibited the proliferation of fibroblasts in vitro in a concentration-dependent manner. The inhibitory effects of dexamethasone and 5-FU were relatively weak, but dexamethasone and other The combined effect of the half-inhibitory concentrations of the three drugs was significantly greater than that of the four drugs alone. CONCLUSION: Daunorubicin and doxorubicin can effectively inhibit rabbit fibroblasts in vitro and suggest that the combination of dexamethasone and dexamethasone may be a valuable medicine for the prevention and treatment of proliferative vitreoretinopathy (PVR)