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利用离体孵育脑薄片和放射免疫测定孵育液内精氨酸升压素（ＡＶＰ）的方法，探讨了皮质酮（Ｂ）及牛血清白蛋白偶联皮质酮（Ｂ－ＢＳＡ）对大鼠下丘脑薄片（含室旁核和视上核）释放ＡＶＰ的快速影响。结果：（１）Ｂ和Ｂ－ＢＳＡ在２０ｍｉｎ内均可明显地抑制ＡＶＰ的释放，在１０－７～１０（－４）ｍｏｌ／Ｌ范围内均呈剂量－效应关系；但在每一剂量，Ｂ－ＢＳＡ的快速抑制效应均弱于Ｂ；（２）ＲＵ４８６对Ｂ和Ｂ－ＢＳＡ的快速抑制效应都有部分的阻断作用；但阻断Ｂ－ＢＳＡ效应的最小剂量比阻断Ｂ者高一个数量级；（３）孵育液Ｃａ~（２＋）浓度升高，Ｂ和Ｂ－ＢＳＡ的快速抑制效应均明显增强；孵育液中无Ｃａ~（２＋）则Ｂ和Ｂ－ＢＳＡ的快速抑制效应有所减弱；但Ｂ－ＢＳＡ效应的变化幅度不及Ｂ大。结果表明：（１）Ｂ与牛血清白蛋白共价偶联后，其快速抑制大鼠下丘脑薄片释放ＡＶＰ的效应有所减弱；（２）Ｂ的快速抑制效应是由非基因组机制介导的，其作用可能是影响Ｃａ~（２＋）跨细胞膜流动的结果。
The effects of corticosterone (B) and bovine serum albumin-conjugated corticosterone (B-BSA) on the expression of arginine vasopressin (AVP) in rat brain were investigated by in vitro incubation of brain slices and radioimmunoassay Thalamic slices (including paraventricular and supraoptic nuclei) release the rapid effects of AVP. Results: (1) The release of AVP was significantly inhibited by B and B-BSA in 20 min, and the dose-response relationship was observed in the range of 10 -7 -10 (-4) mol / L. However, at each dose, B-BSA was weaker than that of B; (2) RU486 partially blocked the rapid inhibitory effect of B and B-BSA; however, the minimum dose of B-BSA was higher than that of B An order of magnitude. (3) The Ca 2+ concentration in the incubation solution increased rapidly and the rapid inhibitory effect of B and B-BSA significantly increased. The rapid inhibitory effect of Ca 2+ -concentration in the incubation solution on B and B-BSA was But the change of B-BSA effect is less than B. The results showed that: (1) B covalently coupled with bovine serum albumin, its rapid inhibition of rat hypothalamic membrane release AVP weakened effect; (2) B rapid inhibitory effect is mediated by non-genomic mechanisms , Which may be the result of affecting Ca ~ (2+) flow across the cell membrane.