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汉防己甲素（汉甲，０．３～３０μｍｏｌ·Ｌ－１）浓度依赖地抑制豚鼠离体气管片和支气管条平滑肌由电场刺激所引起的收缩反应。实验结果显示：汉甲对气道感觉神经Ｃ类纤维兴奋所致收缩的抑制作用强于对胆碱能神经兴奋所致收缩的抑制；上述抑制作用在颈部气管片明显强于胸腔内气管片和支气管条平滑肌。Ｐ＜０．０１（ｂ、ｃ组与ａ组等浓度点的比较）汉甲抑制各组气道平滑肌Ⅰ、Ⅱ相收缩反应的半效抑制量参见附表。附表汉甲抑制豚鼠离休气道平滑肌Ⅰ相和Ⅱ相收缩反应的半效抑制量（ＩＣ５０±９５％可信限）３讨论豚鼠离体气管和支气管标本内神经兴奋时引起平滑肌收缩反应，Ⅰ相收缩是胆碱能神经末梢兴奋引起，Ⅱ相收缩则是感觉神经Ｃ类纤维兴奋所致［１］。汉甲对ＥＦＳ引起的平滑肌收缩的Ⅰ、Ⅱ相反应均有浓度依赖的抑制作用，然而其抑制作用的强度有差别。图１、２的量效曲线和附表资料均显示，汉甲对气道标本Ⅰ相收缩的抑制作用远弱于对Ⅱ相收缩的抑制强度，需较高浓度的汉甲才对１相反应有明显抑制作用，两者的ＩＣ５０值也显示同样的差别。说明汉甲对豚鼠气道中Ｃ类神经纤维兴奋效应的抑制作用明显强于对胆碱能神经兴奋效应的抑制。已有实验证明汉甲通过抑制豚鼠气管平滑肌细胞膜的钙离子通道，?
Tetrandrine (nails, 0.3 ~ 30μmol·L-1) inhibited the contractile response of isolated guinea pig tracheal strips and bronchial smooth muscle stimulated by electric field in a concentration-dependent manner. The results showed that: the inhibitory effect of Nail A on the contraction induced by excitability of C-fiber of airway sensory nerves was stronger than that of the contraction induced by excitability of cholinergic nerve. The inhibition was significantly stronger than that on the tracheal tube And bronchial smooth muscle. P <0.01 (b, c and a group of equal concentration point comparison) Han inhibition of airway smooth muscle Ⅰ, Ⅱ half-inhibitory effect of the contraction phase, see the attached table. Schedule Nail Inhibition of guinea pigs retired airway smooth muscle phase Ⅰ and Ⅱ half-phase inhibition of contraction (IC50 ± 95% confidence limit) 3 guinea pig tracheal and bronchial specimens of nerve excitability induced smooth muscle contraction, Ⅰ Phase contraction is excited by cholinergic nerve endings, Ⅱ phase contraction is sensory nerve fibers caused by C-excited [1]. Nail to EFS-induced contraction of smooth muscle phase Ⅰ, Ⅱ have a concentration-dependent inhibition, however, the intensity of its inhibition vary. Figure 1 and 2 dose-effect curves and schedule data showed that the inhibitory effect of Nail Artemisia Ⅰ contraction of airway specimens is far weaker than that of the inhibition of phase Ⅱ contraction, the higher concentration of hanjia only 1 phase response Have a significant inhibitory effect, the two IC50 values also show the same difference. These results indicated that the inhibitory effect of Nailfruit on the excitatory effects of C-type nerve fibers in the airway of guinea pigs was stronger than that of the excitatory effect of cholinergic nerve. It has been experimentally proved that hanjia through the inhibition of guinea pig tracheal smooth muscle cell membrane calcium channel?