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目的研究鸟巢蕨水提取液对体外培养成骨细胞增殖及分化作用的影响,探讨其促进骨折愈合、防治骨质疏松的机制。方法将不同浓度的鸟巢蕨提取液(分别为0g/L、1.0g/L、1.2g/L、1.4g/L、1.5g/L、1.6g/L、2.0g/L,共7组)与成骨细胞共同体外培养,进行相应的干预。观察细胞形态,检测成骨细胞增殖与分化指标(包括Ⅰ型胶原蛋白、碱性磷酸酶、骨钙素量、钙盐沉积量和矿化结节)。结果与同一时间点的空白组比较,除第1天的1.0g/L组外,其余各组的成骨细胞增殖差异均具有统计学意义(P<0.01)。与空白组比较,其余组别平均光密度值、碱性磷酸酶含量、骨钙素含量、钙沉积量差异均具有统计学意义(P<0.01)。与空白组比较,除1.0g/L组外,其余组别矿化结节数差异均具有统计学意义(P<0.01)。同一时间点1.6g/L组与2.0g/L组比较,成骨细胞增殖差异均无统计学意义(P>0.05);1.6g/L组与2.0g/L组比较,平均光密度值、碱性磷酸酶含量、骨钙素含量、矿化结节数差异无统计学意义(P>0.05),钙沉积量差异具有统计学意义(P<0.01)。结论鸟巢蕨水提取液对成骨细胞的增殖分化具有促进作用,适宜浓度下具有促进成骨细胞增殖分化作用,过高浓度后作用达到饱和。
Objective To study the effect of the water extract of Bird’s Nest Fern on the proliferation and differentiation of osteoblasts cultured in vitro, and explore the mechanism of promoting the fracture healing and preventing osteoporosis. Methods The different concentrations of bird’s nest fern extract (0g/L, 1.0g/L, 1.2g/L, 1.4g/L, 1.5g/L, 1.6g/L, 2.0g/L, respectively, 7 groups) Co-cultured with osteoblasts in vitro and performed appropriate interventions. The morphology of the cells was observed and the osteoblast proliferation and differentiation markers (including type I collagen, alkaline phosphatase, osteocalcin, calcium deposition, and mineralized nodules) were measured. Results Compared with the blank group at the same time point, except for the 1.0 g/L group on the first day, the proliferation of osteoblasts in the other groups had statistical significance (P<0.01). Compared with the blank group, the average optical density, alkaline phosphatase, osteocalcin, and calcium deposition in the other groups were statistically significant (P<0.01). Compared with the blank group, except for the 1.0 g/L group, the number of mineralized nodules in the other groups was statistically significant (P<0.01). There was no significant difference in osteoblast proliferation between the 1.6 g/L group and the 2.0 g/L group at the same time point (P>0.05). The average optical density values were compared between the 1.6 g/L group and the 2.0 g/L group. There was no significant difference in alkaline phosphatase, osteocalcin and mineralized nodule counts (P>0.05). The difference in calcium deposition was statistically significant (P<0.01). Conclusion The water extract of Bird’s Nest Fern can promote the proliferation and differentiation of osteoblasts, and it can promote the proliferation and differentiation of osteoblasts at the appropriate concentration. After high concentration, the effect will reach saturation.