论文部分内容阅读
本文以短枝型苹果首红为试材，以不同的离体脱水时间为处理，测定了１年生枝条不同芽位的芽体和不同枝段组织相对含水量、细胞透性、超过化物歧化酶（ＳＯＤ）活性及同工酶特性、脂质过氧化作用产物丙二醛（ＭＤＡ）含量的变化，结果表明：随着离体脱水时间的加长，各芽体和枝段组织相对含水量均出现明显下降，且不同芽体间和不同枝段间组织相对含水量及其下降幅度不同；细胞透性在离体脱水过程有一个极显著上升，其与组织相对含水量的变化呈显著负相关，Ｒ枝＝－０．９４４１，Ｒ芽＝－０．９０２７；其变化规律呈ｌｏｇｉｓｔｉｃ曲线变化态，配合方程为Ｙ＝Ｋ／（１－ａｅ－ｂｘ），拐点脱水时间值的变化在不同芽体和不同枝段间具有一定差异；随着离体脱水时间加长，芽体中的ＳＯＤ酶活性开始上升（２４ｈ之前）。而后下降（２４ｈ之后），其ＳＯＤ酶聚丙烯酰胺凝胶电泳中酶带数目及级带变化同样说明这一结果；ＭＤＡ含量的变化与ＳＯＤ酶的变化正相反，且具有极显著的相关性Ｒ＝－０．８４１４。
In this paper, spruce apple first red as test material, with different in vitro dehydration time for the treatment, the determination of the buds and branches of different branches of 1-year-old branches relative water content, cell permeability, over the disproportionate enzyme (SOD) activity and isoenzyme characteristics and the content of malondialdehyde (MDA) in the lipid peroxidation products. The results showed that the relative water content of the buds and branches of the buds were significantly increased with the prolongation of in vitro dehydration time Decreased, and the relative water content and the decrease range of the tissue between different buds and different branches were different. The cell permeability had a very significant increase in the process of ex-vivo dehydration, which was significantly negatively correlated with the relative water content of the tissues Branch = -0.9441, R bud = -0.9027. The change law of logarithm was logistic curve with the formula of Y = K / (1-ae-bx) There was a certain difference between different branches. With prolonged ex-vivo dehydration time, SOD activity in buds began to increase (before 24h). And then decreased (after 24h), SOD enzyme polyacrylamide gel electrophoresis in the number of bands and band changes also illustrate this result; changes in MDA content and SOD enzyme changes in the opposite, and has a very significant correlation R = -0.8414.