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人乳头瘤病毒（ＨＰＶ）至今没有合适的体外培养系统。为了研究ＨＰＶ１６Ｅ７蛋白的免疫学性质，采用分子克隆技术构建了我国地方株（湖北株ＨＢ）ＨＰＶ１６Ｅ７基因重组表达质粒。经鉴定显示：该基因在大肠杆菌中得到高效表达，分子量约为６９ＫＤ，为ＨＰＶ１６－ＨＢＥ７与载体蛋白（β－半乳糖苷酶α肽）融合蛋白。融合蛋白表达量占整个菌体蛋白含量的３０％～４０％；Ｗｅｓｔｅｒｎｂｌｏｔ检测结果表明：融合蛋白保留了ＨＰＶ１６Ｅ７抗原决定簇的抗原性，能与Ｅ７单克隆抗体特异性结合。该表达系统的建立为进一步研究Ｅ７蛋白的免疫学性质提供了充足的材料来源
Human papillomavirus (HPV) so far no suitable in vitro culture system. In order to study the immunological properties of HPV16E7 protein, we constructed a recombinant expression plasmid of HPV16E7 gene of our local strain (Hubei strain HB) by molecular cloning technology. It has been identified that the gene is highly expressed in E. coli and has a molecular weight of about 69 kD, which is a fusion protein of HPV16-HBE7 and carrier protein (beta-galactosidase alpha peptide). The fusion protein expressed 30% ~ 40% of the total bacterial protein content; Western blot results showed that: the fusion protein retains HPV16E7 antigenic determinant antigen, with E7 monoclonal antibody specific binding. The establishment of this expression system provided sufficient material sources for further study on the immunological properties of E7 protein