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人类神经tau蛋白在 37℃保温 ,可在 333nm波长形成新荧光峰 ;该荧光分别在 2 30和2 80nm具有最大激发峰 ,其量子产率为 0 .0 2 .该荧光团的形成约需 30h ,同时伴有溶液瑞利散射强度的增加 .肝素对该荧光的形成影响不明显 .以上结果表明 ,333nm新荧光可能来自于tau蛋白分子在溶液中的聚集
The human neurotrophic tau protein was incubated at 37 ° C to form a new fluorescence peak at 333 nm with the maximum excitation peak at 230 and 280 nm and a quantum yield of 0. 02. The fluorophore formation takes about 30 h , Accompanied by an increase in the Rayleigh scattering intensity of the solution.The heparin has no obvious effect on the fluorescence.The above results show that the new fluorescence at 333nm may come from the aggregation of tau protein in solution