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对红江橙上胚轴再生系统建立及影响因素进行了研究，结果表明：上胚轴、带根上胚轴、子叶、下胚轴四种外植体中，上胚轴出芽率、丛生芽所占比例为最高。不同浓度ＢＡ对上胚轴出芽率影响不同，其中ＢＡ２ｍｇ／Ｌ效果最好，出芽率及丛生芽所占比例分别达８２．８％、８６．７％。外植体放置方式影响也较大，主要表现为形态学上端出芽，另一端出芽率很低。抑菌用抗生素中，３００～５００ｍｇ／Ｌ浓度范围内，羧苄青霉素对上胚轴出芽有一定程度抑制；头孢霉素则无影响。上胚轴在培养基上放置方式不同，对卡那霉素的承受能力也不同。竖放时，卡那霉素１６０ｍｇ／Ｌ完全抑制出芽；平放时，５０ｍｇ／Ｌ即完全抑制出芽
The establishment of the regeneration system of the orange hypocotyl and its influencing factors were studied. The results showed that among the four explants, the hypocotyls, the hypocotyls, the cotyledons and the hypocotyls, The highest proportion. Different concentrations of BA had different effects on the budding rate of epicotyls. BA2mg / L had the best effect, and the percentage of budding and budding buds was 82.8% and 86.7% respectively. The explant placement has a greater impact, mainly on morphological budding and low budding on the other. Antibacterial antibiotics, 300 ~ 500mg / L concentration range, carbenicillin on the hypocotyls bud to a certain extent inhibited; cephalosporins have no effect. Hypocotyls placed in different ways on the medium, the tolerance of kanamycin is also different. Vertical, kanamycin 160mg / L completely inhibited budding; laid flat, 50mg / L that is completely inhibited budding