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研究了血小板激活因子（ＰＡＦ）和ＰＡＦ拮抗剂银杏内酯Ｂ对洗涤兔血小板中ｃＡＭＰ含量的作用．结果表明ＰＡＦ（０．１－１．０μｍｏｌ·Ｌ－１）对血小板的基础ｃＡＭＰ水平无影响，但对前列腺素Ｅ１（ＰＧＥ１）２μｍｏｌ·Ｌ－１及４，５－二氢－６－［４－（１Ｈ－咪唑－１－）苯基］－５－甲基－３－（２Ｈ）－哒嗪酮（ＣＩ－９３０）２０μｍｏｌ·Ｌ－１引起的ｃＡＭＰ升高有显著的抑制作用．银杏内酯Ｂ能完全拮抗ＰＡＦ抑制ＰＧＥ１和ＣＩ－９３０升高ｃＡＭＰ的作用，ＩＣ５０分别为４．７和１２．５μｍｏｌ·Ｌ－１．合用磷酸肌酸／磷酸肌酸激酶和阿司匹林对ＰＡＦ和银杏内酯Ｂ的作用均无影响．提示ＰＡＦ对磷酸二酯酶的激活作用及腺苷酸环化酶的抑制作用是ＰＡＦ的直接作用，与其同ＰＡＦ受体结合有关．
The effect of platelet activating factor (PAF) and PAF antagonist ginkgolide B on cAMP content in washed rabbit platelets was investigated. The results showed that PAF (0.1-1.0μmol·L-1) had no effect on basal platelet cAMP level, but no effect on the level of prostaglandin E1 (PGE1) 2μmol·L-1 and 4,5-dihydro-6- [4 - (1H-imidazole-1) phenyl] -5-methyl-3- (2H) -pyridazinone (CI-930) at a concentration of 20 μmol·L -1. Ginkgolide B completely antagonized PAF inhibition of PGE1 and CI-930 increased cAMP, IC50 were 4.7 and 12.5μmol·L-1. Combination of creatine phosphate / creatine phosphokinase and aspirin had no effect on PAF and ginkgolide B effect. These results suggest that the activation of phosphodiesterase by PAF and the inhibitory effect of adenylate cyclase are the direct actions of PAF, which are related to the binding of PAF to PAF.