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目的研究急性肺损伤(ALI)新生儿外周血血清血小板活化因子(PAF)、TNF-α水平变化,探讨PAF、TNF-α在新生儿ALI中的意义。方法采用酶联免疫吸附试验定量测定ALI组、肺炎组及健康对照组新生儿血清PAF、TNF-α水平,并对二者进行相关性分析。结果与肺炎组、健康对照组比较,入院24 h ALI新生儿血清PAF、TNF-α水平均明显增高,差异有统计学意义(Pa<0.01),肺炎组PAF和TNF-α水平与健康对照组比较差异均无统计学意义(Pa>0.05)。ALI组入院24 h、72 h PAF、TNF-α水平比较差异均无统计学意义(Pa>0.05)。PAF与TNF-α水平呈显著正相关(P<0.01)。结论 PAF、TNF-α在新生儿ALI中起重要作用,提示其对新生儿ALI的诊断、治疗有潜在应用价值。
Objective To study the changes of peripheral blood serum platelet-activating factor (PAF) and TNF-α in patients with acute lung injury (ALI) and to explore the significance of PAF and TNF-α in neonatal ALI. Methods Serum levels of PAF and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA) in ALI group, pneumonia group and healthy control group. Correlation analysis was made between the two groups. Results Compared with the pneumonia group and the healthy control group, serum levels of PAF and TNF-α in neonatal ALI neonates were significantly increased 24 h after admission (P <0.01), and the levels of PAF and TNF-α in the pneumonia group were significantly lower than those in the healthy control group There was no significant difference (Pa> 0.05). The levels of PAF and TNF-α in ALI group were not significantly different at 24 h and 72 h (P> 0.05). There was a significant positive correlation between PAF and TNF-α (P <0.01). Conclusions PAF and TNF-α play an important role in neonatal ALI, suggesting that they may have potential value in the diagnosis and treatment of neonatal ALI.