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目的观察母鼠妊娠前接受慢性不可预见性应激(CUS)后皮质醇(COR)水平改变对胎鼠大脑不同区域5-羟色胺1A(5-HT1A)受体表达的影响。方法 SD成年健康雌性大鼠20只随机分为正常对照母鼠组(8只)和CUS母鼠组(12只)。采用11种刺激方法对CUS母鼠组进行21 d CUS,构建CUS模型。造模前后CUS母鼠组和正常对照母鼠组进行开场实验及蔗糖水消耗实验,分析CUS对母鼠自主行为的影响。采用放射免疫法检测妊娠第20天母鼠血清COR水平,分析CUS对母鼠COR的影响。采用反转录-PCR(RT-PCR)法检测胎鼠大脑海马、额前皮质、下丘脑5-HT1A受体mRNA表达情况。结果 1.CUS母鼠组应激后开场实验的总路程、周边路程、中央路程和蔗糖水消耗实验的蔗糖水消耗量明显少于应激前及正常对照母鼠组。2.血清COR水平在正常母鼠组为(155.3±14.4)μg.L-1,CUS组为(190.2±19.8)μg.L-1,2组比较差异有统计学意义(P<0.01)。3.二组胎鼠的体质量和脑质量比较差异均无统计学意义。4.胎鼠海马中5-HT1A受体mRNA的表达量正常对照组为0.286±0.025,CUS组为0.228±0.132,2组比较差异有统计学意义(P<0.01);额前皮质中5-HT1A受体mRNA的表达量正常对照组为0.266±0.028,CUS组为0.249±0.027,2组比较差异有统计学意义(P<0.01);但2组胎鼠下丘脑中5-HT1A受体mRNA表达量比较差异无统计学意义(P>0.05)。结论母鼠妊娠前接受CUS,可引起胎鼠大脑各区域5-HT1A受体表达异常,其机制与母体血清中COR水平有关。实用儿科临床杂志,2011,26(6):404-407
Objective To observe the effect of cortisol (COR) on the expression of 5-hydroxytryptamine 1A (5-HT 1A) receptor in different regions of fetal rat brain after prenatal CUS. Methods Twenty female SD rats were randomly divided into normal control group (n = 8) and female CUS group (n = 12). Eleven kinds of stimulation methods were used to CUS mothers group for 21 d CUS to construct CUS model. Before and after modeling, CUS female rats and normal control rats were subjected to the opening experiment and sucrose water consumption experiment to analyze the influence of CUS on the autonomic behavior of the rats. Radioimmunoassay was used to detect serum COR level on the 20th day of gestation, and the effect of CUS on COR of female rats was analyzed. Reverse transcription-PCR (RT-PCR) was used to detect the mRNA expression of 5-HT1A receptor in fetal rat hippocampus, prefrontal cortex and hypothalamus. Results 1. The total consumption of sucrose water in the course of opening test, the distance traveled, the distance traveled and the sucrose water consumption experiment in the CUS female rats were significantly less than those in the pre-stress and normal control rats. The level of serum COR was (155.3 ± 14.4) μg.L-1 in normal female rats and (190.2 ± 19.8) μg.L-1 in CUS rats, the difference was statistically significant (P <0.01). There was no significant difference in body weight and brain mass between the two groups of fetuses. The expression of 5-HT1A receptor mRNA in the fetal rat hippocampus was 0.286 ± 0.025 in the normal control group and 0.228 ± 0.132 in the CUS group, the difference was statistically significant between the two groups (P <0.01) HT1A receptor mRNA expression in normal control group was 0.266 ± 0.028, CUS group was 0.249 ± 0.027, the difference between the two groups was statistically significant (P <0.01); but 2 groups fetal rat hypothalamus 5-HT1A receptor mRNA There was no significant difference in the expression level (P> 0.05). CONCLUSIONS: CUS received before pregnancy may cause abnormal expression of 5-HT1A receptor in fetal brain regions. The mechanism may be related to COR level in maternal serum. Practical Journal of Pediatrics, 2011,26 (6): 404-407