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目的探讨多囊卵巢综合征(PCOS)脂肪组织脂联素受体AdipoR1(AR1)mRNA表达和3-磷酸肌醇依赖性蛋白激酶-1(PDK1/PDPK1)的蛋白活性在PCOS发病中的作用。方法 PCOS患者和对照者根据体质量指数(BMI)分为PCOS肥胖组、PCOS非肥胖组、肥胖对照组和非肥胖对照组,每组12例,采用RT-PCR技术结合内对照,半定量检测PCOS及其对照组脂肪组织AR1 mRNA的表达,采用脂肪组织蛋白质的提取、SDS-Page、Western blot和增强化学发光蛋白免疫印迹法及图像分析半定量检测脂肪组织PDK1活性。结果脂肪组织中AR1 mRNA的表达量在PCOS肥胖组(0.49±0.13)和PCOS非肥胖组(0.74±0.14)均显著低于非肥胖对照组(0.88±0.15),且以PCOS肥胖组降低更为明显(P<0.001),PCOS肥胖组与肥胖对照组(0.60±0.17)相比差异未见统计学意义(P>0.05)。PDK1蛋白活性在PCOS肥胖组为(55.16±24.37)%,较非肥胖对照组的(84.33±15.54)%明显降低(P<0.001);肥胖对照组为(45.95±22.18)%,PCOS非肥胖组为(71.27±26.42)%,均较非肥胖对照组明显降低(P<0.001和P<0.05),PCOS肥胖组和肥胖对照组之间以及PCOS肥胖组和PCOS非肥胖组之间比较差异均未见统计学意义(P>0.05)。结论 PCOS两组脂肪组织AR1mRNA的表达均较非肥胖对照组降低,且以PCOS肥胖组降低更为明显,PCOS肥胖组和PCOS非肥胖组脂肪组织PDK1活性均较非肥胖对照组明显减弱,低水平的AR1可能通过下调PDK1抑制胰岛素受体后的信号传导,并与PCOS胰岛素抵抗的发生有关。
Objective To investigate the effect of adiponectin receptor AdipoR1 (AR1) mRNA expression and protein kinase activity of 3-phosphoinositide-dependent protein kinase-1 (PDK1 / PDPK1) on the pathogenesis of PCOS in patients with polycystic ovary syndrome (PCOS). Methods PCOS patients and control subjects were divided into PCOS obesity group, PCOS non-obese group, obesity control group and non-obese control group according to body mass index (BMI), 12 cases in each group. RT-PCR combined with internal control and semi-quantitative The expression of AR1 mRNA in adipose tissue of PCOS and its control group was detected semi-quantitatively by using the extraction of protein from adipose tissue, SDS-PAGE, Western blot and western blotting and image analysis. Results The expression of AR1 mRNA in adipose tissue was significantly lower in PCOS obesity group (0.49 ± 0.13) and PCOS non-obese group (0.74 ± 0.14) than in non-obese control group (0.88 ± 0.15), and decreased in PCOS obesity group (P <0.001). There was no significant difference between PCOS obesity group and obese control group (0.60 ± 0.17) (P> 0.05). The activity of PDK1 protein in PCOS obesity group was significantly lower than that in non-obese control group (55.16 ± 24.37% vs 84.33 ± 15.54% vs 45.95 ± 22.18%, PCOS non-obese group (71.27 ± 26.42)%, which were significantly lower than those in the non-obese control group (P <0.001 and P <0.05). There was no significant difference between the PCOS obesity group and the obesity control group and between the PCOS obesity group and the PCOS non-obese group See statistical significance (P> 0.05). Conclusions The expression of AR1 mRNA in adipose tissue of PCOS group is lower than that of non-obese control group, and more obviously in PCOS obesity group. Compared with non-obese control group, PDK1 activity in adipose tissue of PCOS obesity group and PCOS non- Of AR1 may be related to the occurrence of PCOS insulin resistance by down-regulating the signal transduction of PDK1 after inhibiting insulin receptor.