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目的:探讨芹菜素对人肝癌Hepg2细胞增殖与葡萄糖摄取的影响。方法:以Hepg2细胞为研究对象,实验均用0.625~10μmol·L~(-1)不同浓度芹菜素作用于细胞,细胞密度以6×10~3个/m L接种于96孔培养板,5复孔/组,分别培养24,48,72 h后,MTT法检测细胞增殖抑制情况;同时,取96孔板培养24,48,72 h的细胞上清液,用葡萄糖测定试剂盒检测Hepg2细胞葡萄糖摄取情况;细胞密度以10~6个/m L接种于6孔培养板中,培养48 h后,Western blot检测葡萄糖转运蛋白1(GLUT1)表达的变化。结果:与对照组比较,芹菜素各浓度组均能抑制Hepg2细胞的增殖,且抑制作用呈时间和浓度依赖性,差异有统计学意义(P<0.05,P<0.01);芹菜素各浓度组作用后Hepg2细胞对葡萄糖的摄取明显减少(P<0.05,P<0.01);与对照组比较,GLUT1蛋白随着芹菜素浓度的升高表达减少(P<0.05,P<0.01)。结论:芹菜素具有抑制人肝癌Hepg2细胞增殖与葡萄糖摄取的作用,为肝癌的预防和治疗提供新的思路。
Objective: To investigate the effect of apigenin on the proliferation and glucose uptake of HepG2 cells. Methods: HepG2 cells were used as experimental materials. Different concentrations of apigenin (0.625 ~ 10μmol·L -1) were applied to the cells. The cell density was inoculated into 96-well plates at a density of 6 × 10 ~ 3 / The cell proliferation was detected by MTT assay after 24h, 48h and 72h respectively. At the same time, the supernatants of 24, 48 and 72 h were cultured in 96-well plate, and HepG2 cells were detected by glucose assay kit Glucose uptake. The cell density was inoculated into 6-well plates at 10 ~ 6 cells / mL. After cultured for 48 h, the expression of glucose transporter 1 (GLUT1) was detected by Western blot. Results: Compared with the control group, all the concentrations of apigenin could inhibit the proliferation of HepG2 cells in a time and concentration dependent manner (P <0.05, P <0.01) The glucose uptake of Hepg2 cells was significantly decreased after treatment with HepG2 (P <0.05, P <0.01). Compared with the control group, the expression of GLUT1 protein decreased with the increase of apigenin concentration (P <0.05, P <0.01). Conclusion: Apigenin can inhibit the proliferation and glucose uptake of HepG2 cells and provide new ideas for the prevention and treatment of HCC.