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应用兔抗牛丝虫抗体ＥＬＩＳＡ双抗体夹心法和Ｄｏｔ－ＥＬＩＳＡ法检测安徽流行区人体班氏丝虫循环抗原。微丝蚴血症者血清总阳性率９６％（２４／２５），尿液总阳性率为６０％（１５／２５），４份乳汁阳性率均为１００％。１５份尿液阳性和４份乳汁阳性者与血清阳性结果的符合率分别为１００％（１５／１５）和７５％（３／４）。检测江苏非流行区正常人血清、尿液、乳汁共４３份，全部阴性。１１份肠道线虫感染者尿液全为阴性，血清Ｄｏｔ－ＥＬＩＳＡ阴性。１０份肺吸虫感染者血清Ｄｏｔ－ＥＬＩＳＡ阴性。结果表明，班氏丝虫循环抗原不仅存在于血清而且存在于尿液和乳汁中。
Antibodies against rabbits were used to detect the circulating antigens of B. bancrofti in the endemic areas of Anhui Province by double-antibody sandwich ELISA and Dot-ELISA. Microfilaria sepsis serum total positive rate of 96% (24/25), urine total positive rate was 60% (15/25), 4 milk positive rate was 100%. The coincidence rates of 15 urine positive and 4 milk positive patients with seropositive results were 100% (15/15) and 75% (3/4), respectively. A total of 43 serum, urine and milk samples from normal people in non-endemic areas of Jiangsu Province were detected, all negative. Urine was negative in 11 cases of intestinal nematode infection, while serum Dot-ELISA was negative. Serum Dot-ELISA was negative in 10 Paragonimus infection. The results showed that the circulating filaments of Bancroft’s filariasis not only existed in serum but also in urine and milk.