论文部分内容阅读
目的:研究水飞蓟宾对人肺癌A549细胞凋亡的影响。方法:采用MTT法测定水飞蓟宾在不同浓度下对A549细胞的抑制率;分别采用q TR-PCR及Western blot检测细胞中Capase-3、Caspase-9及Bcl-2的mRNA及蛋白表达水平;流式细胞术检测细胞凋亡情况。结果:10、20、40、80、160μg/ml水飞蓟宾对A549细胞的增殖有明显的抑制作用,最高抑制率为46.61±3.70。qRTPCR及Western blot结果显示,与对照组相比,当水飞蓟宾浓度为40~160μg/ml剂量范围时,显著上调Capase-3、Caspase-9的mRNA及蛋白表达水平,下调Bcl-2的mRNA及蛋白表达水平;流式细胞术检测发现,40~160μg/ml剂量范围的水飞蓟宾显著增加了A549细胞的凋亡率。结论:水飞蓟宾能够抑制人肺癌A549细胞株的体外增殖并促进细胞凋亡,其机制可能是通过上调Capase-3、Caspase-9的表达,下调Bcl-2的表达实现的。
Objective: To study the effect of silybin on the apoptosis of human lung cancer A549 cells. Methods: The inhibitory rates of silybin on A549 cells were determined by MTT assay. The mRNA and protein expressions of Capase-3, Caspase-9 and Bcl-2 were detected by qRT-PCR and Western blot respectively Flow cytometry was used to detect apoptosis. Results: Silybin at concentrations of 10, 20, 40, 80 and 160μg / ml significantly inhibited the proliferation of A549 cells with the highest inhibition rate of 46.61 ± 3.70. qRTPCR and Western blot results showed that compared with the control group, when the silybin concentration was 40 ~ 160μg / ml, the mRNA and protein expression of Capase-3 and Caspase-9 were significantly up-regulated and the expression of Bcl-2 The results of flow cytometry showed that silybin significantly increased the apoptosis rate of A549 cells in the dose range of 40 ~ 160μg / ml. Conclusion: Silybin can inhibit the proliferation and promote the apoptosis of human lung cancer cell line A549 in vitro. The possible mechanism is that silybin can down-regulate the expression of Bcl-2 by up-regulating the expression of Capase-3 and Caspase-9.