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从我国分离到的一株单纯疱疹病毒Ⅰ型（ＨＳＶ－１－１６８株）病毒基因组中，分离出含有糖蛋白Ｄ（ｇＤ）基因的１．２ｋｂ片段，插入带有痘苗病毒天坛株ＴＫ区的质粒ｐＪＳＢ１１７５Ｐ７．５ｋ启动子下游，转染无白血病鸡胚原代细胞，获得带有ＨＳＶ－１－１６８ｇＤ基因的重组痘苗病毒。此株重组病毒在感染细胞膜上表达ＨＳＶ－１－１６８ｇＤ糖蛋白抗原，能与特异性单克隆抗体反应。在感染细胞中表达的膜抗原经ＳＤＳ－ＰＡＧＥ分析，表达分子量为５４ｋＤ糖蛋白。用Ｓｏｕｔｈｅｒｎ杂交分析了重组病毒ＤＮＡ中特异的ｇＤ基因，对作为活疫苗的重组痘苗病毒株进行了一些微生物学活性、免疫原性和毒力等方面的研究。
A 1.2kb fragment of glycoprotein D (gD) gene was isolated from a herpes simplex virus type 1 (HSV-1-168) virus genome isolated in China and inserted into the TK region of vaccinated virus Tiantan strain TK Downstream of the plasmid pJSB1175P7.5k promoter, the primary cells of leukemia-free chicken embryos were transfected to obtain the recombinant vaccinia virus with HSV-1-168gD gene. The strain recombinant virus expressed HSV-1-168gD glycoprotein antigen on the infected cell membrane, which could react with specific monoclonal antibody. Membrane antigens expressed in infected cells were analyzed by SDS-PAGE to express a 54 kD glycoprotein. Southern hybridization was used to analyze the specific gD gene in recombinant virus DNA. Some recombinant vaccinia virus strains were tested for their microbiological activity, immunogenicity and virulence.