论文部分内容阅读
目的 探讨米非司酮对早孕绒毛蜕膜细胞凋亡、增殖及相关基因表达的影响。方法 对正常早孕绒毛与蜕膜、药物流产完全与不完全的绒毛、药物流产不完全蜕膜标本各 10份 (共 5 0份标本 ) ,应用原位末端标记法行细胞凋亡的组织学检测 ,免疫组织化学方法测定bcl 2、bax、fas、fasL、增殖细胞核抗原 (PCNA) 5种蛋白在组织中的分布与含量 ,同时应用原位杂交法测定fas与fasLmRNA的分布与含量。结果 正常早孕绒毛中存在少量凋亡细胞 ,主要分布于合体滋养细胞 ,蜕膜中凋亡细胞偶见 ;绒毛蜕膜中均可检测到上述 5种蛋白。米非司酮药物流产的绒毛合体滋养细胞凋亡显著增多 ,蜕膜凋亡细胞也增多 ,同时伴有促凋亡bax、fas、fasL蛋白及fas、fasLmRNA含量的增加 ,而PCNA蛋白含量无减少。结论 米非司酮能促进早孕绒毛合体滋养细胞、蜕膜间质及腺上皮细胞的凋亡 ,且主要通过fas与fasL转录及翻译途径介导 ,bax表达增加也有一定相关性 ,此可能为其抗早孕机理之一。
Objective To investigate the effect of mifepristone on the apoptosis, proliferation and related gene expression of chorionic villi in early chorionic villi. Methods 10 normal ovulation and decidua, complete and incomplete medical abortion, incomplete deciduas of 10 cases (total of 50 specimens) were randomly divided into three groups. Histological examination of apoptosis was performed by in situ end labeling The distributions and contents of bcl-2, bax, fas, fasL and proliferating cell nuclear antigen (PCNA) in tissues were determined by immunohistochemistry. The distribution and content of fas and fasL mRNA in the tissues were also detected by in situ hybridization. Results There were a few apoptotic cells in normal early pregnancy villi, which were mainly distributed in syncytiotrophoblasts and apoptotic cells in decidua occasionally. The above five proteins were detected in chorionic villi. Mifepristone induced abortion of villous syncytiotrophoblast cells increased significantly, decidual cells also increased, accompanied by pro-apoptotic bax, fas, fasL protein and fas, fasLmRNA content increased, while no decrease in PCNA protein content . Conclusion Mifepristone can promote the apoptosis of syncytiotrophoblasts, decidua and glandular epithelial cells in early pregnancy, and it is mainly mediated by the fas and fasL transcription and translation pathway. The increase of bax expression may also be related to it. Anti-early pregnancy mechanism.