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Objectives To investigate the effect and mechanism of endovascular brachytherapy with 192Ir on expression of type I colla gen, metalloproteinases - 1 (MMP - 1) and the tissue inhibitor (TIMP - 1 ) after angioplasty. Methods Restenotic model of domestic microswine was employed and the iliac arteries were randomized to radiation group (n = 12), which were treated with 20 - 25 Gy of 192Ir, and non - radiation group ( n = 36) after angioplasty. The target vessels were harvested in the end of 3 months and 6 months after angioplasty. Im-munohistochemistry and in situ hybridization were used to detect proteins of type I collagen, MMP - 1 and TIMP -1, and mRNA expression of type I collagen. Results The protein and mRNA of type I collagen, the ratios of TIMP-1/MMP-1 were significantly lower in radiation group than in non - radiation group (P < 0. 05 or 0. 01). The peak of transcription of type I collagen mRNA was at 6 months and 3 months in non - radiation group and radiation group respectively. Conclusions Endo
Objectives To investigate the effect and mechanism of endovascular brachytherapy with 192Ir on expression of type I colla gen, metalloproteinases - 1 (MMP - 1) and the tissue inhibitor (TIMP - 1) after angioplasty. Methods Restenotic model of domestic microswine was employed and the iliac arteries were randomized to radiation group (n = 12), which were treated with 20 - 25 Gy of 192Ir, and non - radiation group (n = 36) after angioplasty. The target vessels were harvested in the end of 3 months and 6 months after angioplasty. Im-munohistochemistry and in situ hybridization were used to detect the proteins of type I collagen, MMP-1 and TIMP-1, and mRNA expression of type I collagen. Results The protein and mRNA of type I collagen, the ratios of TIMP-1 / MMP-1 were significantly lower in radiation group than in non-radiation group (P <0.05 or 0.01). The peak of transcription of type I collagen mRNA was at 6 months and 3 months in non- radiation group and radiation group respectively Conclusions Endo