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各种伤病引起的儿童骺板早闭导致肢体严重畸形和功能障碍,是目前小儿矫形外科临床治疗的难题。传统的治疗骨骺提前早闭的方法有骨桥切除后脂肪、多聚硅树脂、骨蜡或骨水泥等填塞方法。近年来,学者们开始探索用组织工程技术修复骺板部分缺损。该文作者应用免疫组化法分析甲壳素支架与骨髓干细胞之间的生物兼容性,探讨应用体外构建的甲壳素支架-骨髓干细胞三维复合体修复兔胫骨大面积骺板缺损的可行性。采用幼兔胫骨近端内侧骺板50％缺损模型,将72只新西兰白兔分为三组(每组24只),A组缺损区不做任何充填,B组充填无细胞的甲壳素支架材料,C组植入甲壳素-骨髓干细胞复合体。通过测量胫骨内、外侧长度差值及股胫
A variety of injuries caused by the epiphyseal plate of children lead to early closure of severe limb deformities and dysfunction, is the clinical treatment of pediatric orthopedic problems. Traditional methods of epiphyseal early closure in advance of the bridge after the removal of fat, silicone, bone wax or bone cement filling method. In recent years, scholars began to explore the use of tissue engineering to repair part of the epiphyseal defects. The authors applied immunohistochemistry to analyze the biocompatibility of chitin scaffolds and bone marrow stem cells and explored the feasibility of using three-dimensional chitin scaffolds - bone marrow stem cells in vitro to repair large epiphyseal defects of tibia in rabbits. Using the 50% defect model of the proximal epiphyseal plate of the tibia in rabbits, 72 New Zealand white rabbits were divided into three groups (24 in each group). The defect in group A was not filled. The group B was filled with cell-free chitin scaffold , C group implanted chitin - bone marrow stem cell complex. By measuring the difference between the medial and lateral tibia and femoral shin