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以受分次全身照射的三种荷瘤小鼠为研究对象,探讨白介素-6(IL-6)对不同肿瘤细胞增殖、凋亡及凋亡相关基因p53、bcl-2表达的影响。本实验采用流式细胞术检测IL-6对S180肉瘤、H22肝癌、Lewis肺癌三种荷瘤小鼠经6MVX线分次全身照射后肿瘤细胞凋亡及增殖指数的影响,以免疫组织抗生蛋白链霉素?过氧化物酶?生物素(SP)法检测以上三种荷瘤小鼠肿瘤组织中p53、bcl-2的表达水平,及IL-6对经照射的三种荷瘤小鼠肿瘤组织p53、bcl-2表达水平的影响。结果表明:在接受全身分次照射的三种荷瘤小鼠中,Lewis肺癌IL-6组SPF值及PI值均较对照组低,AI值较对照组高(P<0.05),S180肉瘤荷瘤鼠结果相反(P<0.01),而在H22肝癌两组肿瘤细胞凋亡及增殖指数未见明显差异(P>0.05)。其机理可能与IL-6改变了肿瘤细胞所处的周期时相的比例有关。IL-6对凋亡相关基因p53、bcl-2的表达在不同肿瘤组织中具有不同影响,Lewis肺癌荷瘤鼠IL-6组肿瘤细胞p53表达水平较对照组低,S180肉瘤荷瘤鼠IL-6组肿瘤细胞bcl-2、p53表达水平较对照组高,而H22肝癌荷瘤鼠两组肿瘤细胞bcl-2、p53表达水平无明显差异。其作用可能与IL-6对不同肿瘤组织中凋亡相关基因p53、bcl-2的表达产生不同影响有关。
To investigate the effect of interleukin-6 (IL-6) on the proliferation and apoptosis of different tumor cells and the expression of apoptosis-related genes p53 and bcl-2 in three kinds of tumor-bearing mice irradiated by divided body. In this study, flow cytometry was used to detect the effect of IL-6 on the apoptosis and proliferation index of tumor cells in S180 sarcoma, H22 hepatoma and Lewis lung carcinoma after 6MVX line irradiation. The levels of p53 and bcl-2 in tumor tissues of the above three kinds of tumor-bearing mice were detected by enzyme-linked immunosorbent assay (ELISA) and peroxidase-biotin (SP) method. The effect of IL-6 on the tumor tissues p53, bcl-2 expression levels. The results showed that the SPF and PI of Lewis lung carcinoma IL-6 group were lower than those of the control group, the AI value was higher (P <0.05) in the three tumor-bearing mice receiving systemic fractional irradiation, the S180 sarcoma burden On the contrary, there was no significant difference between the two groups (P> 0.05). However, there was no significant difference between the two groups (P> 0.05). The mechanism may be related to IL-6 changes in the proportion of tumor cells in the phase of the phase. IL-6 has different effects on the expression of p53 and bcl-2 in different tumor tissues. The expression level of p53 in IL-6 group is lower than that in control group, while S180 sarcoma-bearing mouse IL- The expression levels of bcl-2 and p53 in 6 groups were higher than those in control group, while the expression levels of bcl-2 and p53 in H22 group were not significantly different. It may be related to the different effects of IL-6 on the expression of p53 and bcl-2 in different tumor tissues.