小麦Mlo及NBS-LRR类抗病基因同源序列的分离与鉴定(英文)

• 作 者: 李爱丽 孔秀英 周荣华 马峙英 贾继增
• 机 构: 中国农业科学院作物品种资源研究所,中国农业科学院作物品种资源研究所,中国农业科学院作物品种资源研究所,河北农业大学农学院,中国农业科学院作物品种资源研究所 农业部作物种质资源与生物技术重点开放实验室,
• 出 处: Acta Botanica Sinica
• 发表日期: 2003年04期
• 关键词: Mlo NBS-LRR 近等基因系 抗白粉病基因 白粉病抗性 cDNA克隆 RGA 缺体 控制基因 白粉菌

根据GenBank中公布的大麦白粉病抗性控制基因MlocDNA序列及一个来源于栽培一粒小麦 (TriticummonococcumL .)的假定抗病基因序列分别设计引物 ,以携带小麦抗白粉病基因的近等基因系为材料进行RT_PCR筛选。结果获得两个表达基因的cDNA克隆。其中一个与大麦白粉病抗性控制基因Mlo的同源性达 83%。另一个为非通读序列 ,含有两个可能的开放阅读框 ,分别包含抗病基因NBS保守结构域 2和 3以及与水稻抗稻瘟病基因Pib蛋白末端相似的 1 3个LRR区域 ,推测该序列属于NBS_LRR类。白粉菌诱导前后 ,该片段RT_PCR扩增产物存在差异 ,表明该片段可能与小麦抗病性相关。利用“中国春”缺体_四体系 ,将该NBS_LRR类序列定位在小麦 1D染色体上。

Primers were designed based on the MlocDNA sequence of barley powdery mildew resistance control gene published in GenBank and a putative disease-resistance gene sequence derived from Triticum aestivum. The near-isogenic lines carrying wheat powdery mildew resistance genes were used as materials RT_PCR screening. As a result, two cDNA clones expressing the gene were obtained. One of them shared 83% identity with Mlo, a powdery mildew resistance control gene. The other was a non-read-through sequence containing two possible open reading frames (ORFs) containing the NBS conserved domains 2 and 3 of the disease-resistance gene and 13 LRR regions similar to the end of the Pib protein of the rice blast resistance gene, respectively. NBS_LRR class. Before and after the powdery mildew was induced, there was a difference in the RT_PCR amplification products of the fragment, indicating that the fragment may be related to the disease resistance of wheat. The NBS_LRR sequence was mapped on chromosome 1D in wheat using the “Chinese Spring”