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该文探讨了胃癌细胞培养液上清对人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,huc MSCs)向癌相关MSCs转分化的作用及转分化后的MSCs对胃癌细胞增殖和迁移的影响。收集胃癌细胞HGC-27的培养液上清,与等体积的低糖DMEM混合后培养huc MSCs 48 h,检测处理后huc MSCs中癌相关MSCs蛋白及mi R-221表达的改变。用诱导后的huc MSCs培养液上清与等体积的高糖DMEM混合后培养HGC-27 48 h,检测处理前后HGC-27的增殖和迁移能力的变化。将huc MSCs和HGC-27共培养,检测共培养后HGC-27增殖和迁移能力。结果发现,胃癌细胞培养液上清处理后,huc MSCs中波形蛋白(vimentin)和成纤维细胞活化蛋白(fibroblast activation protein,FAP)表达增强,mi R-221表达上调。用处理后的huc MSCs培养液上清培养HGC-27,细胞增殖以及迁移能力增强。共培养后HGC-27的增殖及迁移能力显著增强。以上结果表明,胃癌细胞培养液上清可诱导huc MSCs获得癌相关MSCs样表型和促进胃癌细胞增殖和迁移的功能,为肿瘤微环境细胞的重塑性和脐带MSCs应用的安全性评估提供实验依据。
This study investigated the effect of gastric cancer cell culture supernatant on the transdifferentiation of human umbilical cord mesenchymal stem cells (huc MSCs) into cancer-associated MSCs and the effect of transplanted MSCs on the proliferation and migration of gastric cancer cells . The supernatant of gastric cancer cell line HGC-27 was collected and mixed with an equal volume of low-sugar DMEM. The cultured MSCs were cultured for 48 h. The expression of cancer-associated MSCs and mi R-221 in the huc MSCs were detected. HGC-27 cells were cultured for 48 h after being mixed with an equal volume of high glucose DMEM supernatant and the changes of proliferation and migration ability of HGC-27 before and after treatment were detected. The cocultures of huc MSCs and HGC-27 were co-cultured to detect the proliferation and migration of HGC-27 after co-culture. The results showed that the expression of vimentin and fibroblast activation protein (FAP) in huc MSCs was enhanced and the expression of mi R-221 was up-regulated after gastric cancer cell culture supernatant treatment. HGC-27 was cultured in the supernatant of the treated huc MSCs, and cell proliferation and migration were enhanced. After co-culture HGC-27 proliferation and migration ability was significantly enhanced. The above results show that the gastric cancer cell culture supernatant can induce huc MSCs to obtain cancer-associated MSCs-like phenotype and promote the proliferation and migration of gastric cancer cells, to provide experimental evidence for tumor microenvironmental cell remodeling and umbilical cord MSCs safety assessment in accordance with.