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应用 SLAB 免疫组织化学方法,针对 c-erbB-2 p185癌基因蛋白与慢性乙型肝炎肝组织免疫损伤的关系,在以下方面进行了初步探索。资料与方法1.资料115例 HBV 感染半年以上患者,男86例,女29例,大多年龄20～35岁(占58.3%),全部经肝病理组织学诊断,计慢性迁延性肝炎(CPH)65例,慢性活动性肝炎(CAH)41例,未见明显肝组织异常及仅表现轻微非特异肝组织改变(ASC)9例。以血清HBsAg 和肝组织 HBV DNA 阳性的肝细胞癌55份、肝硬化29份及血清 HBV 指标阴性的正常肝组织6份为对照标本。2.方法所有肝穿标本经10%甲醛固定,石蜡包埋,作连续切片,厚4～5μm。分别作 HE 和免疫组织化学染色,进行病理组织学和
The application of SLAB immunohistochemical method for the c-erbB-2 p185 oncogene protein and chronic hepatitis B liver tissue injury, the relationship between the following aspects of the initial exploration. Materials and Methods Data 115 cases of HBV infection in patients more than six months, 86 males and 29 females, mostly aged 20 to 35 years (58.3%), all diagnosed by histopathology, including chronic persistent hepatitis (CPH) 65 cases, 41 cases of chronic active hepatitis (CAH), no obvious liver abnormalities and only showed slight non-specific liver tissue changes (ASC) in 9 cases. Serum HBsAg and liver tissue HBV DNA-positive hepatocellular carcinoma in 55, 29 cirrhosis and serum HBV-negative normal liver tissue 6 samples for the control. 2. Methods All liver specimens were fixed with 10% formaldehyde and embedded in paraffin for serial sectioning, thickness 4 ~ 5μm. HE and immunohistochemical staining were performed respectively, histopathology and